Reversible control of cell membrane receptor function using DNA nano-spring multivalent ligands,Chemical Science

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Reversible control of cell membrane receptor function using DNA nano-spring multivalent ligands
Chemical Science ( IF 7.6 ) Pub Date : 2017-08-18 00:00:00 , DOI: 10.1039/c7sc02489d
Kaixiang Zhang _{1,

2,

3,

4,

5} , Ruijie Deng _{1,

2,

3,

4,

5} , Yupeng Sun _{1,

2,

3,

4,

5} , Ling Zhang _{1,

2,

3,

4,

5} , Jinghong Li _{1,

2,

3,

4,

5}

Affiliation

Chemically functionalized and nanostructured materials, which mimic the features of the natural extracellular matrix, provide a tool to organize cell surface receptors into nanoscale clusters and manipulate cell functions. However, the existing materials are mainly based on static structures. Herein, we developed a DNA based structure-switchable and multivalent material that acts as a ‘nano-spring’, enabling reversible control of membrane receptor function at the cell surface. This ‘nano-spring’ can be easily synthesized by rolling circle amplification and finely tuned by changing the circular template design. Using this ‘nano-spring’ to interact with cells, we have demonstrated that the movement of the DNA nanostructure is sufficient to direct a cell morphology change from the normal morphology to having numerous cell protrusions and affect the mRNA expression level of integrin related genes. This DNA nano-spring structure can be a competitive material for actively manipulating cell receptor function and may help us to understand the role of receptor mediated signalling cascades.

中文翻译：

使用DNA纳米弹簧多价配体可逆地控制细胞膜受体功能

化学功能化和纳米结构的材料模仿了天然细胞外基质的特征，提供了一种将细胞表面受体组织成纳米级簇并操纵细胞功能的工具。但是，现有材料主要基于静态结构。在本文中，我们开发了一种基于DNA的结构可转换的多价材料，可充当“纳米弹簧”，从而可逆地控制细胞表面的膜受体功能。这种“纳米弹簧”可以通过滚圆放大轻松合成，并可以通过更改圆形模板设计进行微调。利用这种“纳米弹簧”与细胞相互作用，我们已经证明，DNA纳米结构的运动足以指导细胞形态从正常形态转变为具有许多细胞突起，并影响整联蛋白相关基因的mRNA表达水平。这种DNA纳米弹簧结构可以成为主动调控细胞受体功能的竞争性材料，并且可以帮助我们了解受体介导的信号级联反应的作用。

更新日期：2017-09-25

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