A red-emissive antibody–AIEgen conjugate for turn-on and wash-free imaging of specific cancer cells,Chemical Science

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A red-emissive antibody–AIEgen conjugate for turn-on and wash-free imaging of specific cancer cells
Chemical Science ( IF 7.6 ) Pub Date : 2017-08-17 00:00:00 , DOI: 10.1039/c7sc01054k
Xiujuan Shi _{1,

2,

3,

4,

5} , Chris Y. Y. Yu _{1,

2,

3,

4,

5} , Huifang Su _{1,

2,

3,

4,

5} , Ryan T. K. Kwok _{1,

2,

3,

4,

5} , Meijuan Jiang _{1,

2,

3,

4,

5} , Zikai He _{1,

2,

3,

4,

5} , Jacky W. Y. Lam _{1,

2,

3,

4,

5} , Ben Zhong Tang _{1,

2,

3,

4,

5}

Affiliation

An antibody–AIEgen conjugate is designed and developed as a “turn-on” fluorescent probe for wash-free specific cancer cell imaging. The cetuximab-conjugated AIEgen shows red fluorescence only when it is internalized and accumulated in cancer cells with overexpressed epidermal growth factor receptor through endocytosis. The probe first lights up the lysosomes. After hydrolysis, its residue is accumulated in mitochondria, making them highly emissive with a long cell retention time. Compared with conventional “always-on” fluorescent probes, the antibody–AIEgen conjugate exhibits a very good image contrast during wash-free cancer cell imaging and less interference from normal cells. To the best of our knowledge, this is the first time “turn-on” antibody–AIEgen conjugates have been reported. This new strategy can be further extended to many proteins and water-soluble AIEgens, and many of their potential applications such as real-time tracking of cell dynamics and cancer theranostics will be explored. The present work is expected to inspire more marvellous research in the fields of AIE and cancer imaging.

中文翻译：

红发射抗体-AIEgen共轭物，用于特定癌细胞的开启和免洗成像

抗体-AIEgen偶联物被设计和开发为“免洗”特异性癌细胞成像的“开启”荧光探针。结合西妥昔单抗的AIEgen仅在通过内吞作用被内化并积聚在表皮生长因子受体过表达的癌细胞中时才会显示红色荧光。探针首先点亮溶酶体。水解后，其残留物堆积在线粒体中，使其具有高发射性，并具有较长的细胞保留时间。与传统的“永远在线”荧光探针相比，抗体-AIEgen偶联物在免洗癌细胞成像过程中显示出非常好的图像对比度，并且对正常细胞的干扰较小。据我们所知，这是首次报道“开启”抗体-AIEgen偶联物。这种新的策略可以进一步扩展到许多蛋白质和水溶性AIEgens，并且将探索它们的许多潜在应用，例如实时跟踪细胞动力学和癌症治疗学。预期当前的工作将激发AIE和癌症成像领域的更多精彩研究。

更新日期：2017-09-25

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