The development of rapid analytical strategies plays a vital role for the research, discovery and confirmation of analytes that can be sensitive biomarkers. Within this line of work, a method based on headspace-gas chromatography-mass spectrometry has been developed and validated for the determination of possible biomarkers (dimethyl disulfide, toluene, 1,4-xylene, 2-octanone and 2,6-dimethyl-7-octen-2-ol) in human urine samples. The matrix effect was analyzed and the optimal analytical conditions were determined. The limits of detection ranged from 0.01 to 0.48 µg L-1, whereas the limits of quantification ranged from 0.05 to 1.43 µg L-1. The repeatability and reproducibility of the proposed method were below 9.5 % and 14.2 %, respectively. Five urine samples from healthy subjects, spiked with the possible biomarkers, were used to validate the proposed methodology. The quantification of these compounds in three urine samples from healthy subjects was performed with the one-point standard additions protocol.

中文翻译：

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顶空-气相色谱-质谱法快速测定尿液样品中可能的生物标志物

快速分析策略的发展对于可能是敏感生物标志物的分析物的研究，发现和确认起着至关重要的作用。在这一工作范围内，已开发出一种基于顶空-气相色谱-质谱联用的方法，并经过验证可用于确定可能的生物标志物（二甲基二硫，甲苯，1,4-二甲苯，2-辛酮和2,6-二甲基-人尿液样本中的7-辛烯-2-醇）。分析基质效应并确定最佳分析条件。检测极限范围为0.01至0.48 µg L-1，而定量极限范围为0.05至1.43 µg L-1。该方法的重复性和重现性分别低于9.5％和14.2％。健康受试者的五个尿液样本中掺入了可能的生物标志物，被用来验证所提出的方法。使用一点标准添加方案对健康受试者的三个尿液样品中的这些化合物进行定量。