One-step detection of microRNA with high sensitivity and specificity via target-triggered loop-mediated isothermal amplification (TT-LAMP),Chemical Communications

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One-step detection of microRNA with high sensitivity and specificity via target-triggered loop-mediated isothermal amplification (TT-LAMP)
Chemical Communications ( IF 4.9 ) Pub Date : 2017-09-18 00:00:00 , DOI: 10.1039/c7cc06140d
Yuanyuan Sun _{1,

2,

3,

4,

5} , Hui Tian _{1,

2,

3,

4,

5} , Chenghui Liu _{1,

2,

3,

4,

5} , Yueying Sun _{1,

2,

3,

4,

5} , Zhengping Li _{1,

2,

3,

4,

5}

Affiliation

A novel one-step microRNA assay is developed based on a target-triggered loop-mediated isothermal amplification (TT-LAMP) mechanism, which enables the accurate detection of as low as 100 aM (1 zmol) microRNA with simple one-step operation by using only one-type of DNA polymerase.

中文翻译：

通过靶标触发的环介导的等温扩增（TT-LAMP）一步一步检测高灵敏度和特异性的microRNA

基于目标触发的环介导的等温扩增（TT-LAMP）机制，开发了一种新颖的一步microRNA检测方法，该方法可通过简单的一步操作即可准确检测低至100 aM（1 zmol）的microRNA。仅使用一种类型的DNA聚合酶。

更新日期：2017-09-22

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