Single quantum dot-based nanosensor for rapid and sensitive detection of terminal deoxynucleotidyl transferase,Chemical Communications

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Single quantum dot-based nanosensor for rapid and sensitive detection of terminal deoxynucleotidyl transferase
Chemical Communications ( IF 4.3 ) Pub Date : 2017-09-14 00:00:00 , DOI: 10.1039/c7cc05485h
Li-Juan Wang _{1,

2,

3,

4,

5} , Ming-Li Luo _{1,

2,

3,

4,

5} , Qianyi Zhang _{6,

7,

8} , Bo Tang _{1,

2,

3,

4,

5} , Chun-Yang Zhang _{1,

2,

3,

4,

5}

Affiliation

We developed a simple and rapid method for terminal deoxynucleotidyl transferase (TdT) assay on the basis of the polymerization-directed exonuclease-assisted construction of a single quantum dot (QD)-based fluorescence resonance energy transfer (FRET) nanosensor. This method is very sensitive with a detection limit as low as 1 × 10⁻⁶ U μL⁻¹, and it can be used for the screening of TDT inhibitors and accurate quantification of TdT activity even in 5 cancer cells.

中文翻译：

基于单量子点的纳米传感器，用于快速灵敏地检测末端脱氧核苷酸转移酶

我们开发了一种简单快速的末端脱氧核苷酸转移酶（TdT）分析方法，该方法基于聚合定向核酸外切酶辅助的基于单个量子点（QD）的荧光共振能量转移（FRET）纳米传感器的构建。该方法非常敏感，检出限低至1×10 ^-6 UμL ^-1，即使在5个癌细胞中，也可用于TDT抑制剂的筛选和TdT活性的准确定量。

更新日期：2017-09-22

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