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Multifunctional (3-in-1) cancer theranostics applications of hydroxyquinoline-appended polyfluorene nanoparticles
Chemical Science ( IF 7.6 ) Pub Date : 2017-08-29 00:00:00 , DOI: 10.1039/c7sc03321d Sayan Roy Chowdhury 1, 2, 3, 4 , Sudip Mukherjee 4, 5, 6, 7, 8 , Sourav Das 4, 5, 6, 7, 8 , Chitta Ranjan Patra 4, 5, 6, 7, 8 , Parameswar Krishnan Iyer 1, 2, 3, 4, 9
Chemical Science ( IF 7.6 ) Pub Date : 2017-08-29 00:00:00 , DOI: 10.1039/c7sc03321d Sayan Roy Chowdhury 1, 2, 3, 4 , Sudip Mukherjee 4, 5, 6, 7, 8 , Sourav Das 4, 5, 6, 7, 8 , Chitta Ranjan Patra 4, 5, 6, 7, 8 , Parameswar Krishnan Iyer 1, 2, 3, 4, 9
Affiliation
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The accumulation of fluorescent hydroxyquinoline-affixed polyfluorene (PF-HQ) nanoparticles and their utility for multi-color bio-imaging and drug delivery for cancer treatment are reported. The formation of nanoparticles (PF-HQ) containing hydrophobic pockets via three-dimensional growth of a polymeric backbone in a higher water fraction (THF
:H2O = 1:9) was observed. The nanoparticles showed incredible dual-state optical and fluorescence properties, which were further explored in multi-color cell imaging in both cancer and normal cells. The cell viability assay in various normal cells confirmed the biocompatible nature of PF-HQ, which was further supported by an ex vivo (chick chorioallantoic membrane assay) model. This encouraged us to fabricate PF-HQ-based new drug delivery systems (DDS: PF-HQ–DOX) upon conjugation with the FDA-approved anti-cancer drug doxorubicin (DOX) by filling the hydrophobic pockets of the polymer nanoparticles. The enhanced anti-cancer activity of the DDS (PF-HQ–DOX) compared with that of free DOX was observed in mouse melanoma cancer cells (B16F10) and a subcutaneous mouse (C57BL6/J) melanoma tumor model upon administration of PF-HQ–DOX. Ex vivo biodistribution studies using a fluorescence quantification method demonstrated the enhanced accumulation of DOX in tumor tissues in the PF-HQ–DOX-treated group compared to that of the free drug, signifying the drug delivery efficacy of the delivery system by a passive targeting manner. Based on the above biological data (in vitro and in the pre-clinical model), these robust and versatile fluorescent hydroxyquinoline-affixed polyfluorene (PF-HQ) nanoparticles could be effectively utilized for multifunctional biomedical applications (as they are biocompatible and can be used for bio-imaging and as a drug delivery vehicle).
中文翻译：
羟基喹啉附加的聚芴纳米粒子的多功能（3合1）癌症治疗学应用
报告了荧光羟基喹啉固定的聚芴（PF-HQ）纳米粒子的积累及其在多色生物成像和药物治疗癌症中的效用。观察到通过在较高水分数（THF：H 2 O = 1 ：9）中聚合物主链的三维生长，形成了包含疏水性口袋的纳米颗粒（PF-HQ）。纳米粒子显示出令人难以置信的双态光学和荧光性质，这在癌症和正常细胞的多色细胞成像中得到了进一步探索。在各种正常细胞中的细胞活力测定证实了PF-HQ的生物相容性，这在离体体内得到了进一步的支持。（小鸡绒膜尿囊膜测定）模型。这鼓励我们通过填充聚合物纳米颗粒的疏水性口袋，与FDA批准的抗癌药物阿霉素（DOX）结合，制造出基于PF-HQ的新药物递送系统（DDS：PF-HQ-DOX）。施用PF-HQ后，在小鼠黑素瘤癌细胞（B16F10）和皮下小鼠（C57BL6 / J）黑色素瘤肿瘤模型中观察到DDS（PF-HQ-DOX）与游离DOX相比具有增强的抗癌活性。 –DOX。离体使用荧光定量方法进行的生物分布研究表明，与游离药物相比，PF-HQ-DOX治疗组的肿瘤组织中DOX的积累有所增强，这表明该传递系统通过被动靶向方式的给药效率。基于上述生物学数据（在体外和临床前模型中），这些坚固耐用且用途广泛的荧光羟基喹啉固定的聚芴（PF-HQ）纳米颗粒可有效地用于多功能生物医学应用（因为它们具有生物相容性并且可以使用用于生物成像和作为药物输送工具）。
更新日期：2017-09-22
:H2O = 1:9) was observed. The nanoparticles showed incredible dual-state optical and fluorescence properties, which were further explored in multi-color cell imaging in both cancer and normal cells. The cell viability assay in various normal cells confirmed the biocompatible nature of PF-HQ, which was further supported by an ex vivo (chick chorioallantoic membrane assay) model. This encouraged us to fabricate PF-HQ-based new drug delivery systems (DDS: PF-HQ–DOX) upon conjugation with the FDA-approved anti-cancer drug doxorubicin (DOX) by filling the hydrophobic pockets of the polymer nanoparticles. The enhanced anti-cancer activity of the DDS (PF-HQ–DOX) compared with that of free DOX was observed in mouse melanoma cancer cells (B16F10) and a subcutaneous mouse (C57BL6/J) melanoma tumor model upon administration of PF-HQ–DOX. Ex vivo biodistribution studies using a fluorescence quantification method demonstrated the enhanced accumulation of DOX in tumor tissues in the PF-HQ–DOX-treated group compared to that of the free drug, signifying the drug delivery efficacy of the delivery system by a passive targeting manner. Based on the above biological data (in vitro and in the pre-clinical model), these robust and versatile fluorescent hydroxyquinoline-affixed polyfluorene (PF-HQ) nanoparticles could be effectively utilized for multifunctional biomedical applications (as they are biocompatible and can be used for bio-imaging and as a drug delivery vehicle).
中文翻译：
羟基喹啉附加的聚芴纳米粒子的多功能（3合1）癌症治疗学应用
报告了荧光羟基喹啉固定的聚芴（PF-HQ）纳米粒子的积累及其在多色生物成像和药物治疗癌症中的效用。观察到通过在较高水分数（THF
：H 2 O = 1 ：9）中聚合物主链的三维生长，形成了包含疏水性口袋的纳米颗粒（PF-HQ）。纳米粒子显示出令人难以置信的双态光学和荧光性质，这在癌症和正常细胞的多色细胞成像中得到了进一步探索。在各种正常细胞中的细胞活力测定证实了PF-HQ的生物相容性，这在离体体内得到了进一步的支持。（小鸡绒膜尿囊膜测定）模型。这鼓励我们通过填充聚合物纳米颗粒的疏水性口袋，与FDA批准的抗癌药物阿霉素（DOX）结合，制造出基于PF-HQ的新药物递送系统（DDS：PF-HQ-DOX）。施用PF-HQ后，在小鼠黑素瘤癌细胞（B16F10）和皮下小鼠（C57BL6 / J）黑色素瘤肿瘤模型中观察到DDS（PF-HQ-DOX）与游离DOX相比具有增强的抗癌活性。 –DOX。离体使用荧光定量方法进行的生物分布研究表明，与游离药物相比，PF-HQ-DOX治疗组的肿瘤组织中DOX的积累有所增强，这表明该传递系统通过被动靶向方式的给药效率。基于上述生物学数据（在体外和临床前模型中），这些坚固耐用且用途广泛的荧光羟基喹啉固定的聚芴（PF-HQ）纳米颗粒可有效地用于多功能生物医学应用（因为它们具有生物相容性并且可以使用用于生物成像和作为药物输送工具）。
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