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Design of a Dual Channel Self-Reference Photoelectrochemical Biosensor
Analytical Chemistry ( IF 6.7 ) Pub Date : 2017-09-22 00:00:00 , DOI: 10.1021/acs.analchem.7b03132 Nan Hao 1 , Ying Zhang 1 , Hui Zhong 1 , Zhou Zhou 1 , Rong Hua 1 , Jing Qian 1 , Qian Liu 1 , Henan Li 1 , Kun Wang 1
Analytical Chemistry ( IF 6.7 ) Pub Date : 2017-09-22 00:00:00 , DOI: 10.1021/acs.analchem.7b03132 Nan Hao 1 , Ying Zhang 1 , Hui Zhong 1 , Zhou Zhou 1 , Rong Hua 1 , Jing Qian 1 , Qian Liu 1 , Henan Li 1 , Kun Wang 1
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Photoelectrochemical (PEC) biosensors are usually based on the single photocurrent change caused by biorecognition events between analytes and probes. However, the photocurrent may be influenced by other factors besides target analytes and bring a false result. To improve the accuracy and reliability of PEC detection, here we proposed the design of a dual channel self-reference PEC biosensors. CdTe and CdTe-graphene oxide (GO) were chosen as the two PEC active material and modified onto two adjacent areas on the ITO electrode. Then they were functionalized with Aflatoxin B1 (AFB1) aptamer through covalent binding or physical adsorption, respectively. The cathodic current from CdTe-GO and anodic current from CdTe can be well distinguished by adjusting the bias voltage. With the simultaneous application of “signal on” and “signal off” model, dual concentration information may be obtained in one detection and serve as a reference for each other. By comparing these two results, this sensor can clearly distinguish whether the signal change was caused by AFB1 or other interference factors. Compared to traditional PEC biosensors, this design can provide a better accuracy and reliability, which is promising in the future development of PEC detection.
中文翻译：
双通道自参考光电化学生物传感器的设计
光电化学（PEC）生物传感器通常基于由分析物和探针之间的生物识别事件引起的单个光电流变化。但是，光电流可能会受目标分析物以外的其他因素影响，并带来错误的结果。为了提高PEC检测的准确性和可靠性，我们在此提出了一种双通道自参考PEC生物传感器的设计。选择CdTe和CdTe氧化石墨烯（GO）作为两种PEC活性材料，并将其改性到ITO电极上的两个相邻区域上。然后将它们分别通过共价结合或物理吸附分别用黄曲霉毒素B1（AFB1）适体进行功能化。通过调节偏置电压，可以很好地区分来自CdTe-GO的阴极电流和来自CdTe的阳极电流。在同时使用“信号开启”和“信号关闭”模型的情况下，在一次检测中可以获得双重浓度信息，并且可以相互参考。通过比较这两个结果，此传感器可以清楚地区分信号变化是由AFB1还是其他干扰因素引起的。与传统的PEC生物传感器相比，该设计可以提供更好的准确性和可靠性，这在PEC检测的未来发展中很有希望。
更新日期：2017-09-22
中文翻译：
双通道自参考光电化学生物传感器的设计
光电化学（PEC）生物传感器通常基于由分析物和探针之间的生物识别事件引起的单个光电流变化。但是，光电流可能会受目标分析物以外的其他因素影响，并带来错误的结果。为了提高PEC检测的准确性和可靠性，我们在此提出了一种双通道自参考PEC生物传感器的设计。选择CdTe和CdTe氧化石墨烯（GO）作为两种PEC活性材料，并将其改性到ITO电极上的两个相邻区域上。然后将它们分别通过共价结合或物理吸附分别用黄曲霉毒素B1（AFB1）适体进行功能化。通过调节偏置电压，可以很好地区分来自CdTe-GO的阴极电流和来自CdTe的阳极电流。在同时使用“信号开启”和“信号关闭”模型的情况下，在一次检测中可以获得双重浓度信息，并且可以相互参考。通过比较这两个结果，此传感器可以清楚地区分信号变化是由AFB1还是其他干扰因素引起的。与传统的PEC生物传感器相比，该设计可以提供更好的准确性和可靠性，这在PEC检测的未来发展中很有希望。
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