Nanomolar Level Detection of Uric Acid in Blood Serum and Pest-Infested Grain Samples by an Amphiphilic Probe,Analytical Chemistry

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Nanomolar Level Detection of Uric Acid in Blood Serum and Pest-Infested Grain Samples by an Amphiphilic Probe
Analytical Chemistry ( IF 6.7 ) Pub Date : 2017-09-21 00:00:00 , DOI: 10.1021/acs.analchem.7b02344
Nilanjan Dey ₁ , Santanu Bhattacharya ₁

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A pyrene based amphiphilic receptor has been utilized in the nanomolar detection of uric acid at physiological pH in water. The compound shows a unique concentration-dependent modulation in optical response toward uric acid. In intramolecular/static excimer form (low concentration range), it displays a ratiometric response, while a “turn-off” sensing is observed specifically in the presence of intermolecular/dynamic excimer (high concentration range). The present protocol is then employed for the estimation of uric acid in blood serum samples of healthy individuals. Bland-Altman analysis in comparison to clinically approved uricase assay indicated the high accuracy of the present method. Additionally, the extent of insect infestation in stored grain samples was determined by measuring the uric acid content of their aqueous extracts. Low-cost color strips were developed for on-site detection of uric acid without involving any sophisticated instrument or trained personnel.

中文翻译：

两亲探针检测血清和病虫害谷物样品中尿酸的纳摩尔水平

基于pyr的两亲受体已被用于水中生理pH值下尿酸的纳摩尔检测。该化合物在对尿酸的光学响应中显示出独特的浓度依赖性调制。在分子内/静态准分子形式下（低浓度范围），它显示出比例响应，而在分子间/动态准分子下（高浓度范围内）则特别观察到“关闭”感测。然后，将本方案用于估计健康个体的血清样品中的尿酸。与临床批准的尿酸酶测定法相比，Bland-Altman分析表明了本方法的高精度。另外，通过测量水提取物中尿酸含量来确定储存的谷物样品中的昆虫侵染程度。

更新日期：2017-09-21

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