Visualizing Breast Cancer Cell Proliferation and Invasion for Assessing Drug Efficacy with a Fluorescent Nanoprobe,Analytical Chemistry

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Visualizing Breast Cancer Cell Proliferation and Invasion for Assessing Drug Efficacy with a Fluorescent Nanoprobe
Analytical Chemistry ( IF 6.7 ) Pub Date : 2017-09-21 00:00:00 , DOI: 10.1021/acs.analchem.7b03146
Mingming Luan ₁ , Longhai Yu ₁ , Yanhua Li ₁ , Wei Pan ₁ , Xiaonan Gao ₁ , Xiuyan Wan ₁ , Na Li ₁ , Bo Tang ₁

Affiliation

Hard-to-treat cancers are closely relative to uncontrolled cell proliferation, invasion, and metastasis. Assessing proliferation and invasion properties of tumor cells both in vitro and in vivo is especially important for acquiring reliable information for cancer pathogenesis, drug screening, and therapeutic effect evaluation. Herein, we developed a multicolor fluorescent nanoprobe for simultaneously monitoring breast cancer cells’ proliferation marker Ki-67 and invasion marker urokinase plasminogen activator (uPA). After treated with the anticancer drugs tamoxifen and curcumin, the changes in cancer cell proliferation and invasion properties were visually detected and therapeutic effects of corresponding drugs were further assessed in vitro and in vivo. The design of the fluorescent nanoprobe opens up an avenue for investigating unscheduled proliferation, invasion, and metastasis in living cells and in vivo and as such will be a promising tool to screen antitumor drugs and evaluate drug efficiency in an extremely efficient manner.

中文翻译：

用荧光纳米探针可视化乳腺癌细胞的增殖和侵袭，以评估药物疗效

难以治疗的癌症与不受控制的细胞增殖，侵袭和转移密切相关。在体外和体内评估肿瘤细胞的增殖和侵袭特性对于获得可靠的信息以用于癌症发病机理，药物筛选和治疗效果评估尤其重要。本文中，我们开发了一种多色荧光纳米探针，用于同时监测乳腺癌细胞的增殖标记Ki-67和侵袭标记尿激酶纤溶酶原激活剂（uPA）。用抗癌药物他莫昔芬和姜黄素治疗后，肉眼观察到癌细胞增殖和侵袭特性的变化，并进一步评估了相应药物的体外和体外治疗效果。体内。荧光纳米探针的设计为研究活细胞和体内非计划的增殖，侵袭和转移开辟了一条途径，因此将成为筛选抗肿瘤药物并以极其有效的方式评估药物效率的有前途的工具。

更新日期：2017-09-21

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