There has been a growing interest in mitophagy, mitochondria-selective autophagy, which plays an essential role in maintaining intracellular homeostasis. We have developed a small-molecule fluorescent probe, Mtphagy Dye, for visualizing mitophagy, which was readily synthesized from a known perylene derivative, perylene-3,4-dicarboxylic anhydride. Mtphagy Dye has suitable fluorescent properties for detecting mitochondrial acidification during mitophagy in the long-wavelength region that does not damage mitochondria. Using Mtphagy Dye, we were able to visualize mitophagy with both cases of Parkin-dependent and -independent HeLa cells.

中文翻译：

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新型荧光小分子线粒体自噬的活细胞成像

对线粒体，线粒体选择性自噬的研究越来越浓厚，它在维持细胞内稳态方面起着至关重要的作用。我们已经开发了一种用于可视化线粒体的小分子荧光探针Mtphagy Dye，它很容易从已知的per衍生物，per 3,4-二羧酸酐合成。Mtphagy染料具有合适的荧光特性，可在不破坏线粒体的长波长区域中检测线粒体过程中的线粒体酸化。使用Mtphagy染料，我们能够观察到帕金依赖型和非依赖型HeLa细胞的线粒体现象。