Quantitative analysis of different nitrogen isotope labelled nitrates in paddy soil using mid-infrared attenuated total reflectance spectroscopy,Analytical Methods

当前位置： X-MOL 学术 › Anal. Methods › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Quantitative analysis of different nitrogen isotope labelled nitrates in paddy soil using mid-infrared attenuated total reflectance spectroscopy
Analytical Methods ( IF 2.7 ) Pub Date : 2017-08-21 00:00:00 , DOI: 10.1039/c7ay01507k
Yanqiu Shao _{1,

2,

3,

4,

5} , Changwen Du _{4,

5,

6,

7,

8} , Jianmin Zhou _{4,

5,

6,

7,

8} , Fei Ma _{4,

5,

6,

7,

8} , Ying Zhu _{1,

2,

3,

4} , Kai Yang _{1,

2,

3,

4} , Chao Tian _{1,

2,

3,

4}

Affiliation

Nitrogen isotope labelled technology with mass spectroscopy is commonly used to trace the fate of agricultural N in the environment. However, due to mass spectroscopy's cost, time consumption and long laborious preparation, Fourier transform infrared attenuated total reflection spectroscopy (FTIR-ATR) was employed to detect different isotope labelled nitrates in this study, and the feasibility of this method was investigated in paddy soil. The results showed that the RPD of a partial least squares regression (PLSR) model were 5.69 and 8.15 mg kg⁻¹ for ¹⁴NO₃–N and ¹⁵NO₃–N, respectively, and the RMSEP were 5.60 and 3.91 mg kg⁻¹, respectively, which indicated that the method could well predict the nitrate concentration. The model implementation indicated that the total nitrate concentrations from FTIR-ATR and mass spectrometry were almost the same, among which ¹⁵N labelled nitrate obtained by ATR was 3.58–10.02 mg kg⁻¹ lower than that from mass spectrometry, while ¹⁴N labelled nitrate from ATR was 6.75–13.68 mg kg⁻¹ higher. Compared with a control treatment, the mineralization and release of soil nitrogen were enhanced in a nitrogen treatment. Therefore, the technique of FTIR-ATR can be used as an alternative option in determining isotope labelled nitrate in paddy soil.

中文翻译：

中红外衰减全反射光谱法定量分析水稻土中不同氮同位素标记的硝酸盐

氮同位素标记质谱技术通常用于追踪环境中农业氮的命运。然而，由于质谱法的成本，时间消耗和准备工作的繁琐，本研究采用傅立叶变换红外衰减全反射光谱法（FTIR-ATR）检测不同同位素标记的硝酸盐，并研究了该方法在稻田土壤中的可行性。。结果显示，偏最小二乘回归（PLSR）模型的RPD分别为¹⁴ NO ₃ -N和¹⁵ NO ₃ -N的RPD分别为5.69和8.15 mg kg ^-1，RMSEP分别为5.60和3.91 mg kg ^-1分别表明该方法可以很好地预测硝酸盐浓度。该模型的实施表明，FTIR-ATR和质谱法测定的总硝酸盐浓度几乎相同，其中ATR法得到的¹⁵ N标记硝酸盐比质谱法测定的要低3.58-10.02 mg kg ^-1，而¹⁴ N标记硝酸盐含量低。ATR的排放量增加了6.75–13.68 mg kg ^-1。与对照处理相比，氮处理提高了土壤氮的矿化和释放。因此，FTIR-ATR技术可以用作测定水稻土中同位素标记的硝酸盐的替代选择。

更新日期：2017-09-21

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11