In recent years, high demands on food safety technology have made precision and accurate detection technology an urgent research topic. This paper presents a novel biochemical system prototype for colony counting of Escherichia coli using a nano-probe associated with adenosine triphosphate (ATP). The novelty of this system is that the magnetic nanoparticle microbeads are modified with specific antibodies as a probe to capture and enrich the E. coli (Escherichia coli) via the specific connection between antigen and antibody. An experimental test-prototype has been developed in which the light is emitted by an ATP bioluminescence mechanism, the light signal is transformed into an electric signal through a photoelectric multiplier tube, and the quantity of E. coli can be rapidly detected indirectly by a signal processing module. For a testing period of less than twenty minutes, the detectable bacteria concentration range reaches 10²–10⁸ CFU mL⁻¹. Under optimal testing conditions, the limit of detection (LOD) is only 3.0 × 10² CFU mL⁻¹. The data measured in independent experiments show that the maximum fluctuation value of the SNR (signal to noise ratio) is below 6.98% which demonstrates that the prototype has excellent reliability and repeatability. The signal processing module consists of a high-precision chopper amplifier with differential magnification up to 3010 times and a SNR less than 50 dB. Furthermore, the linear correlation coefficient of the prototype is 0.964. It can be seen that the system performance is significant.

中文翻译：

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使用纳米探针和ATP生物发光技术快速检测食品中的大肠杆菌

近年来，对食品安全技术的高要求已使精密和准确的检测技术成为紧迫的研究课题。本文介绍了一种新型的生化系统原型，用于使用与三磷酸腺苷（ATP）结合的纳米探针对大肠杆菌进行菌落计数。该系统的新颖性在于，磁性纳米颗粒微珠被特异性抗体修饰，可作为探针捕获和富集大肠杆菌（Escherichia coli）。抗原和抗体之间的特定联系。已经开发出一种实验测试原型，其中通过ATP生物发光机制发出光，通过光电倍增管将光信号转换为电信号，并且可以通过信号间接地快速检测大肠杆菌的量。处理模块。在不到二十分钟的测试时间内，可检测到的细菌浓度范围达到10 ² –10 ⁸ CFU mL ^-1。在最佳测试条件下，检出限（LOD）仅为3.0×10 ² CFU mL ^-1。在独立实验中测得的数据表明，SNR的最大波动值（信噪比）低于6.98％，这表明该原型具有出色的可靠性和可重复性。信号处理模块由一个高精度斩波放大器组成，其差分放大率高达3010倍，SNR小于50 dB。此外，原型的线性相关系数为0.964。可以看出，系统性能是显着的。