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An efficient grafting technique for producing molecularly imprinted film via reversible addition–fragmentation chain transfer polymerization
Analytical Methods ( IF 2.7 ) Pub Date : 2017-08-14 00:00:00 , DOI: 10.1039/c7ay01623a Y. Wang 1, 2, 3, 4 , S. Q. Jiao 2, 3, 4, 5 , X. L. Chen 2, 3, 4, 5 , T. X. Wei 2, 3, 4, 5
Analytical Methods ( IF 2.7 ) Pub Date : 2017-08-14 00:00:00 , DOI: 10.1039/c7ay01623a Y. Wang 1, 2, 3, 4 , S. Q. Jiao 2, 3, 4, 5 , X. L. Chen 2, 3, 4, 5 , T. X. Wei 2, 3, 4, 5
Affiliation
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Herein, an efficient approach towards obtaining molecularly imprinted polymer (MIP) film for the detection of 17β-estradiol (E2) with water-compatible properties using a reversible addition–fragmentation chain transfer (RAFT) via photo-initiation is described. 2-Methyl-2-[(dodecylsulfanylthiocarbonyl)sulfanyl]propanoic acid (DDMAT) as the sole control agent in the absence of additional photoinitiators or catalysts was utilized for the synthesis of MIP film. The MIP film was characterized using contact angle measurements, infrared spectroscopy (IR) and scanning electron microscopy (SEM). Analysis of the surface plasmon resonance (SPR) spectra showed that the imprinted film displayed good sensitivity and selectivity to E2 when compared to other competitors and non-imprinted polymer (NIP) film. The response of the E2 ranged from 10−14 to 10−6 mol L−1 with a limit of detection (LOD) of 1.15 × 10−15 mol L−1 (S/N = 3) in PBS buffer (pH 7.4). This sensor showed good reusability and stability, and retained about 84.8% of its original response after 45 days of storage under dry and ambient conditions. Finally, the sensor was applied to determine the concentration of E2 in tap water and underground water samples in the range from 10−13 mol L−1 to 10−7 mol L−1, and the LODs were obtained as 7.4 × 10−15 mol L−1 and 1.12 × 10−14 mol L−1, respectively.
中文翻译：
通过可逆加成-断裂链转移聚合生产分子印迹膜的有效接枝技术
在此，向用于使用可逆加成-断裂链转移（RAFT）与水相容的特性的检测17β雌二醇（E2）的获得分子印迹聚合物（MIP）膜中的有效的方法通过描述了光引发。在没有另外的光引发剂或催化剂的情况下，将2-甲基-2-[（十二烷基硫烷基硫代羰基硫烷基）硫烷基]丙酸（DDMAT）作为唯一的控制剂用于MIP膜的合成。使用接触角测量，红外光谱（IR）和扫描电子显微镜（SEM）对MIP膜进行表征。表面等离振子共振（SPR）光谱分析表明，与其他竞争者和非印迹聚合物（NIP）膜相比，印迹膜对E2表现出良好的敏感性和选择性。E2的响应范围为10 -14至10 -6 mol L -1，检测极限（LOD）为1.15×10 -15 mol L -1（S / N = 3）在PBS缓冲液（pH 7.4）中。该传感器显示出良好的可重用性和稳定性，并且在干燥和环境条件下储存45天后，保留了其原始响应的约84.8％。最后，应用该传感器确定自来水和地下水样品中E2的浓度在10 -13 mol L -1至10 -7 mol L -1范围内，LOD为7.4×10 -15 mol L -1和1.12×10 -14 mol L -1。
更新日期：2017-09-21
中文翻译：
通过可逆加成-断裂链转移聚合生产分子印迹膜的有效接枝技术
在此，向用于使用可逆加成-断裂链转移（RAFT）与水相容的特性的检测17β雌二醇（E2）的获得分子印迹聚合物（MIP）膜中的有效的方法通过描述了光引发。在没有另外的光引发剂或催化剂的情况下，将2-甲基-2-[（十二烷基硫烷基硫代羰基硫烷基）硫烷基]丙酸（DDMAT）作为唯一的控制剂用于MIP膜的合成。使用接触角测量，红外光谱（IR）和扫描电子显微镜（SEM）对MIP膜进行表征。表面等离振子共振（SPR）光谱分析表明，与其他竞争者和非印迹聚合物（NIP）膜相比，印迹膜对E2表现出良好的敏感性和选择性。E2的响应范围为10 -14至10 -6 mol L -1，检测极限（LOD）为1.15×10 -15 mol L -1（S / N = 3）在PBS缓冲液（pH 7.4）中。该传感器显示出良好的可重用性和稳定性，并且在干燥和环境条件下储存45天后，保留了其原始响应的约84.8％。最后，应用该传感器确定自来水和地下水样品中E2的浓度在10 -13 mol L -1至10 -7 mol L -1范围内，LOD为7.4×10 -15 mol L -1和1.12×10 -14 mol L -1。
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