Immobilized enzymes: understanding enzyme – surface interactions at the molecular level,Organic & Biomolecular Chemistry

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Immobilized enzymes: understanding enzyme – surface interactions at the molecular level
Organic & Biomolecular Chemistry ( IF 2.9 ) Pub Date : 2017-09-13 00:00:00 , DOI: 10.1039/c7ob01880k
Marie Hoarau _{1,

2,

3,

4} , Somayesadat Badieyan _{1,

2,

3,

4} , E. Neil G. Marsh _{1,

2,

3,

4,

5}

Affiliation

Enzymes immobilized on solid supports have important and industrial and medical applications. However, their uses are limited by the significant reductions in activity and stability that often accompany the immobilization process. Here we review recent advances in our understanding of the molecular level interactions between proteins and supporting surfaces that contribute to changes in stability and activity. This understanding has been facilitated by the application of various surface-sensitive spectroscopic techniques that allow the structure and orientation of enzymes at the solid/liquid interface to be probed, often with monolayer sensitivity. An appreciation of the molecular interactions between enzyme and surface support has allowed the surface chemistry and method of enzyme attachement to be fine-tuned such that activity and stability can be greatly enhanced. These advances suggest that a much wider variety of enzymes may eventually be amenable to immobilization as green catalysts.

中文翻译：

固定化酶：在分子水平上了解酶与表面的相互作用

固定在固体支持物上的酶具有重要的工业和医学应用。然而，它们的使用受到固定过程中经常伴随的活性和稳定性的显着降低的限制。在这里，我们回顾了对蛋白质与支持表面之间的分子水平相互作用的了解方面的最新进展，这些相互作用有助于稳定性和活性的变化。通过应用各种表面敏感的光谱技术可以促进这种理解，这些技术通常以单层敏感性来允许探测固体/液体界面处酶的结构和方向。对酶和表面载体之间的分子相互作用的认识使得可以对酶的表面化学性质和方法进行微调，从而可以大大提高活性和稳定性。这些进展表明，更多种类的酶最终可能适合作为绿色催化剂固定化。

更新日期：2017-09-21

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