A multi-functional guanine derivative for studying the DNA G-quadruplex structure,Analyst

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A multi-functional guanine derivative for studying the DNA G-quadruplex structure
Analyst ( IF 3.6 ) Pub Date : 2017-09-01 00:00:00 , DOI: 10.1039/c7an00941k
Takumi Ishizuka _{1,

2,

3,

4,

5} , Pei-Yan Zhao _{1,

2,

3,

4,

5} , Hong-Liang Bao _{1,

2,

3,

4,

5} , Yan Xu _{1,

2,

3,

4,

5}

Affiliation

In the present study, we developed a multi-functional guanine derivative, ^8FG, as a G-quadruplex stabilizer, a fluorescent probe for the detection of G-quadruplex formation, and a ¹⁹F sensor for the observation of the G-quadruplex. We demonstrate that the functional nucleoside bearing a 3,5-bis(trifluoromethyl)benzene group at the 8-position of guanine stabilizes the DNA G-quadruplex structure and fluoresces following the G-quadruplex formation. Furthermore, we show that the functional sensor can be used to directly observe DNA G-quadruplexes by ¹⁹F-NMR in living cells. To our knowledge, this is the first study showing that the nucleoside derivative simultaneously allows for three kinds of functions at a single G-quadruplex DNA. Our results suggest that the multi-functional nucleoside derivative can be broadly used for studying the G-quadruplex structure and serves as a powerful tool for examining the molecular basis of G-quadruplex formation in vitro and in living cells.

中文翻译：

用于研究DNA G-四链体结构的多功能鸟嘌呤衍生物

在本研究中，我们开发了多功能鸟嘌呤衍生物^8F G，作为G-四链体稳定剂，用于检测G-四链体形成的荧光探针和用于观察G-四链体的¹⁹ F传感器。我们证明，在鸟嘌呤的8位上带有3,5-双（三氟甲基）苯基的功能性核苷可稳定DNA G-四链体结构，并在G-四链体形成后发出荧光。此外，我们表明功能传感器可用于直接观察到¹⁹年前的DNA G-四链体活细胞中的F-NMR。据我们所知，这是第一项表明核苷衍生物同时在单个G-四链体DNA上具有三种功能的研究。我们的结果表明，多功能核苷衍生物可广泛用于研究G-四链体的结构，并作为检查体外和在活细胞中G-四链体形成的分子基础的有力工具。

更新日期：2017-09-21

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