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Large-scale immuno-magnetic cell sorting of T cells based on a self-designed high-throughput system for potential clinical application
Nanoscale ( IF 5.8 ) Pub Date : 2017-08-23 00:00:00 , DOI: 10.1039/c7nr04914e Qian Zhang 1, 2, 3, 4, 5 , Ting Yin 1, 2, 3, 4, 5 , Rongrong Xu 6, 7, 8, 9, 10 , Wenjun Gao 3, 4, 5, 10, 11 , Hui Zhao 3, 4, 5, 10, 11 , Joseph G. Shapter 12, 13, 14, 15 , Kan Wang 1, 2, 3, 4, 5 , Yulan Shen 10, 16, 17, 18, 19 , Peng Huang 1, 2, 3, 4, 5 , Guo Gao 1, 2, 3, 4, 5 , Yanfeng Wu 6, 7, 8, 9, 10 , Daxiang Cui 1, 2, 3, 4, 5
Nanoscale ( IF 5.8 ) Pub Date : 2017-08-23 00:00:00 , DOI: 10.1039/c7nr04914e Qian Zhang 1, 2, 3, 4, 5 , Ting Yin 1, 2, 3, 4, 5 , Rongrong Xu 6, 7, 8, 9, 10 , Wenjun Gao 3, 4, 5, 10, 11 , Hui Zhao 3, 4, 5, 10, 11 , Joseph G. Shapter 12, 13, 14, 15 , Kan Wang 1, 2, 3, 4, 5 , Yulan Shen 10, 16, 17, 18, 19 , Peng Huang 1, 2, 3, 4, 5 , Guo Gao 1, 2, 3, 4, 5 , Yanfeng Wu 6, 7, 8, 9, 10 , Daxiang Cui 1, 2, 3, 4, 5
Affiliation
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In this contribution, we designed four types of immuno-magnetic nanoparticles for separation of different T cells (CD3+, CD4+, CD8+ and CD14+ T cells), and we established a new large-scale immuno-magnetic cell sorting procedure to achieve an enrichment of particular T cells using our designed auto-IMACS device. This device could achieve recyclable large-scale cell sorting, for which the throughput of the system reached ∼4000 mL and the maximum cell capacity was 4 × 1010. The collected cells were analyzed by flow cytometry and visual cytology data, and the effective selection rates of CD3+, CD4+, CD8+ and CD14+ T cells were 79.3%, 74.1%, 57.1% and 67.9%, respectively. The sorted CD8+ T cells still retained good cytotoxic activity against specific cells. In addition, the sorted T cells can also be further incubated in vitro and proliferated, and even could be infused back into patients for immunotherapy in the near future.
中文翻译：
基于自行设计的高通量系统的T细胞大规模免疫磁性细胞分选，可用于潜在临床应用
在这项贡献中，我们设计了四种类型的免疫磁性纳米颗粒用于分离不同的T细胞（CD3 +，CD4 +，CD8 +和CD14 + T细胞），并建立了一种新的大规模免疫磁性细胞分选程序来使用我们设计的自动IMACS设备可实现特定T细胞的富集。该设备可以实现可回收的大规模细胞分选，系统的通量达到约4000 mL，最大细胞容量为4×10 10。通过流式细胞术和视觉细胞学数据分析收集的细胞，以及CD3 +，CD4 +，CD8 +和CD14 +的有效选择率T细胞分别为79.3％，74.1％，57.1％和67.9％。分选的CD8 + T细胞仍然对特定细胞保持良好的细胞毒活性。另外，分选的T细胞还可以在体外进一步温育并增殖，甚至可以在不久的将来输回患者体内进行免疫治疗。
更新日期：2017-09-21
中文翻译：
基于自行设计的高通量系统的T细胞大规模免疫磁性细胞分选，可用于潜在临床应用
在这项贡献中，我们设计了四种类型的免疫磁性纳米颗粒用于分离不同的T细胞（CD3 +，CD4 +，CD8 +和CD14 + T细胞），并建立了一种新的大规模免疫磁性细胞分选程序来使用我们设计的自动IMACS设备可实现特定T细胞的富集。该设备可以实现可回收的大规模细胞分选，系统的通量达到约4000 mL，最大细胞容量为4×10 10。通过流式细胞术和视觉细胞学数据分析收集的细胞，以及CD3 +，CD4 +，CD8 +和CD14 +的有效选择率T细胞分别为79.3％，74.1％，57.1％和67.9％。分选的CD8 + T细胞仍然对特定细胞保持良好的细胞毒活性。另外，分选的T细胞还可以在体外进一步温育并增殖，甚至可以在不久的将来输回患者体内进行免疫治疗。
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