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Nanostructured interfacial self-assembled peptide–polymer membranes for enhanced mineralization and cell adhesion
Nanoscale ( IF 5.8 ) Pub Date : 2017-08-16 00:00:00 , DOI: 10.1039/c7nr03410e Sofia Ribeiro 1, 2, 3, 4, 5 , Elham Radvar 6, 7, 8, 9, 10 , Yejiao Shi 6, 7, 8, 9, 10 , João Borges 11, 12, 13, 14, 15 , Rogério P. Pirraco 1, 2, 3, 4, 5 , Isabel B. Leonor 1, 2, 3, 4, 5 , João F. Mano 11, 12, 13, 14, 15 , Rui L. Reis 1, 2, 3, 4, 5 , Álvaro Mata 6, 7, 8, 9, 10 , Helena S. Azevedo 1, 2, 3, 4, 5
Nanoscale ( IF 5.8 ) Pub Date : 2017-08-16 00:00:00 , DOI: 10.1039/c7nr03410e Sofia Ribeiro 1, 2, 3, 4, 5 , Elham Radvar 6, 7, 8, 9, 10 , Yejiao Shi 6, 7, 8, 9, 10 , João Borges 11, 12, 13, 14, 15 , Rogério P. Pirraco 1, 2, 3, 4, 5 , Isabel B. Leonor 1, 2, 3, 4, 5 , João F. Mano 11, 12, 13, 14, 15 , Rui L. Reis 1, 2, 3, 4, 5 , Álvaro Mata 6, 7, 8, 9, 10 , Helena S. Azevedo 1, 2, 3, 4, 5
Affiliation
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Soft interfacial materials, such as self-assembled polymer membranes, are gaining increasing interest as biomaterials since they can provide selective barriers and/or controlled affinity interactions important to regulate cellular processes. Herein, we report the design and fabrication of multiscale structured membranes integrating selective molecular functionalities for potential applications in bone regeneration. The membranes were obtained by interfacial self-assembly of miscible aqueous solutions of hyaluronan and multi-domain peptides (MDPs) incorporating distinct biochemical motifs, including mineralizing (EE), integrin-binding (RGDS) and osteogenic (YGFGG) peptide sequences. Circular dichroism and Fourier transform infrared spectroscopy analyses of the MDPs revealed a predominant β-sheet conformation, while transmission electron microscopy (TEM) showed the formation of fibre-like nanostructures with different lengths. Scanning electron microscopy (SEM) of the membranes showed an anisotropic structure and surfaces with different nanotopographies, reflecting the morphological differences observed under TEM. All the membranes were able to promote the deposition of a calcium-phosphate mineral on their surface when incubated in a mineralizing solution. The ability of the MDPs, coated on coverslips or presented within the membranes, to support cell adhesion was investigated using primary adult periosteum-derived cells (PDCs) under serum-free conditions. Cells on the membranes lacking RGDS remained round, while in the presence of RGDS they appear to be more elongated and anchored to the membrane. These observations were confirmed by SEM analysis that showed cells attached to the membrane and exhibiting an extended morphology with close interactions with the membrane surface. We anticipate that these molecularly designed interfacial membranes can both provide relevant biochemical signals and structural biomimetic components for stem cell growth and differentiation and ultimately promote bone regeneration.
中文翻译：
纳米结构的自组装肽-聚合物膜，可增强矿化作用和细胞粘附
柔软的界面材料（例如自组装聚合物膜）作为生物材料越来越受到关注，因为它们可以提供对调节细胞过程重要的选择性屏障和/或受控的亲和力相互作用。在这里，我们报告设计和制造集成了潜在的骨再生应用选择性分子功能的多尺度结构化膜。该膜是通过透明质酸和具有不同生化基序的多域肽（MDP）的可混溶水溶液的界面自组装而获得的，这些独特的生化基序包括矿化（EE），整联蛋白结合（RGDS）和成骨（YGFGG）肽序列。MDP的圆二色性和傅立叶变换红外光谱分析表明，β-片层构象占优势，透射电子显微镜（TEM）显示了不同长度的纤维状纳米结构的形成。膜的扫描电子显微镜（SEM）显示出各向异性的结构和具有不同纳米形貌的表面，反映了在TEM下观察到的形态学差异。当在矿化溶液中孵育时，所有的膜都能够促进磷酸钙矿物质在其表面上的沉积。在无血清条件下，使用原代成年骨膜来源的细胞（PDC）研究了涂在盖玻片上或存在于膜中的MDP支持细胞粘附的能力。缺少RGDS的膜上的细胞仍然是圆形的，而在存在RGDS的情况下，它们似乎更细长并锚定在膜上。通过SEM分析证实了这些观察结果，SEM分析显示细胞附着在膜上并表现出扩展的形态，并与膜表面紧密相互作用。我们预期这些分子设计的界面膜可以为干细胞生长和分化提供相关的生化信号和结构仿生成分，并最终促进骨再生。
更新日期：2017-09-21
中文翻译：
纳米结构的自组装肽-聚合物膜，可增强矿化作用和细胞粘附
柔软的界面材料（例如自组装聚合物膜）作为生物材料越来越受到关注，因为它们可以提供对调节细胞过程重要的选择性屏障和/或受控的亲和力相互作用。在这里，我们报告设计和制造集成了潜在的骨再生应用选择性分子功能的多尺度结构化膜。该膜是通过透明质酸和具有不同生化基序的多域肽（MDP）的可混溶水溶液的界面自组装而获得的，这些独特的生化基序包括矿化（EE），整联蛋白结合（RGDS）和成骨（YGFGG）肽序列。MDP的圆二色性和傅立叶变换红外光谱分析表明，β-片层构象占优势，透射电子显微镜（TEM）显示了不同长度的纤维状纳米结构的形成。膜的扫描电子显微镜（SEM）显示出各向异性的结构和具有不同纳米形貌的表面，反映了在TEM下观察到的形态学差异。当在矿化溶液中孵育时，所有的膜都能够促进磷酸钙矿物质在其表面上的沉积。在无血清条件下，使用原代成年骨膜来源的细胞（PDC）研究了涂在盖玻片上或存在于膜中的MDP支持细胞粘附的能力。缺少RGDS的膜上的细胞仍然是圆形的，而在存在RGDS的情况下，它们似乎更细长并锚定在膜上。通过SEM分析证实了这些观察结果，SEM分析显示细胞附着在膜上并表现出扩展的形态，并与膜表面紧密相互作用。我们预期这些分子设计的界面膜可以为干细胞生长和分化提供相关的生化信号和结构仿生成分，并最终促进骨再生。
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