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Graphite particles induce ROS formation in cell free systems and human cells
Nanoscale ( IF 5.8 ) Pub Date : 2017-08-08 00:00:00 , DOI: 10.1039/c7nr02540h G. Zerbi 1, 2, 3, 4, 5 , A. Barbon 5, 6, 7 , R. Bengalli 5, 8, 9 , A. Lucotti 1, 2, 3, 4, 5 , T. Catelani 5, 10, 11, 12 , F. Tampieri 5, 6, 7 , M. Gualtieri 5, 13, 14, 15 , M. D'Arienzo 5, 9, 16 , F. Morazzoni 5, 9, 16 , M. Camatini 5, 8, 9
Nanoscale ( IF 5.8 ) Pub Date : 2017-08-08 00:00:00 , DOI: 10.1039/c7nr02540h G. Zerbi 1, 2, 3, 4, 5 , A. Barbon 5, 6, 7 , R. Bengalli 5, 8, 9 , A. Lucotti 1, 2, 3, 4, 5 , T. Catelani 5, 10, 11, 12 , F. Tampieri 5, 6, 7 , M. Gualtieri 5, 13, 14, 15 , M. D'Arienzo 5, 9, 16 , F. Morazzoni 5, 9, 16 , M. Camatini 5, 8, 9
Affiliation
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It is commonly accepted that the toxicity of carbonaceous particulate matter (PM) is due to the production of reactive oxygen species (ROS) which induce biological damage in the exposed cells. It is also known that PM produced during the combustion processes consists of a carbonaceous core “dressed” with other organic and/or inorganic materials. In spite of this knowledge, the role of these materials in the production of ROS has not yet been clear. This work aims at understanding whether “naked” carbonaceous particles are capable of forming ROS either in cell-free or in-cell systems. The problem has been treated based on the data collected from pure graphite samples of different sizes obtained by ball-milling pure graphite for various lengths of time. The experimental approach considered Raman, ESR (spin trapping), cell viability and fluorescence spectroscopy measurements. These techniques allowed us to carry out measurements both in cell and cell-free systems and the results consistently indicate that also pure naked carbonaceous particles can catalyze the electron transfer that produces superoxide ions. The process depends on the particle size and enlightens the role of the edges of the graphitic platelets. Evidence has been collected that even “naked” graphitic nanoparticles are capable of producing ROS and decreasing the cell viability thus representing a potential danger to human health.
中文翻译：
石墨颗粒诱导无细胞系统和人体细胞中ROS的形成
普遍接受的是，碳质颗粒物（PM）的毒性是由于活性氧（ROS）的产生，该活性氧会在暴露的细胞中引起生物损伤。还已知在燃烧过程中产生的PM由与其他有机和/或无机材料“修饰”的碳质芯组成。尽管有这些知识，但这些材料在生产ROS中的作用尚不清楚。这项工作旨在了解“裸露”的碳质颗粒是否能够在无细胞或细胞内系统中形成ROS。该问题已根据通过球磨纯石墨不同时间长度而获得的不同大小的纯石墨样品收集的数据进行了处理。实验方法考虑了拉曼，ESR（自旋陷阱），细胞活力和荧光光谱测量。这些技术使我们能够在无细胞和无细胞系统中进行测量，结果一致表明纯净的裸露碳质颗粒也可以催化产生超氧离子的电子转移。该过程取决于颗粒大小，并启发石墨薄片边缘的作用。已有证据表明，即使是“裸”石墨纳米颗粒也能够产生ROS并降低细胞活力，从而对人体健康构成潜在威胁。该过程取决于颗粒大小，并启发石墨薄片边缘的作用。已有证据表明，即使是“裸”石墨纳米颗粒也能够产生ROS并降低细胞活力，从而对人体健康构成潜在威胁。该过程取决于颗粒大小，并启发石墨薄片边缘的作用。已有证据表明，即使是“裸”石墨纳米颗粒也能够产生ROS并降低细胞活力，从而对人体健康构成潜在威胁。
更新日期：2017-09-21
中文翻译：
石墨颗粒诱导无细胞系统和人体细胞中ROS的形成
普遍接受的是，碳质颗粒物（PM）的毒性是由于活性氧（ROS）的产生，该活性氧会在暴露的细胞中引起生物损伤。还已知在燃烧过程中产生的PM由与其他有机和/或无机材料“修饰”的碳质芯组成。尽管有这些知识，但这些材料在生产ROS中的作用尚不清楚。这项工作旨在了解“裸露”的碳质颗粒是否能够在无细胞或细胞内系统中形成ROS。该问题已根据通过球磨纯石墨不同时间长度而获得的不同大小的纯石墨样品收集的数据进行了处理。实验方法考虑了拉曼，ESR（自旋陷阱），细胞活力和荧光光谱测量。这些技术使我们能够在无细胞和无细胞系统中进行测量，结果一致表明纯净的裸露碳质颗粒也可以催化产生超氧离子的电子转移。该过程取决于颗粒大小，并启发石墨薄片边缘的作用。已有证据表明，即使是“裸”石墨纳米颗粒也能够产生ROS并降低细胞活力，从而对人体健康构成潜在威胁。该过程取决于颗粒大小，并启发石墨薄片边缘的作用。已有证据表明，即使是“裸”石墨纳米颗粒也能够产生ROS并降低细胞活力，从而对人体健康构成潜在威胁。该过程取决于颗粒大小，并启发石墨薄片边缘的作用。已有证据表明，即使是“裸”石墨纳米颗粒也能够产生ROS并降低细胞活力，从而对人体健康构成潜在威胁。
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