A new model using a gold nanoparticle (AuNP)–DNA system to constrain leakage and improve efficiency of catalytic toehold-mediated strand displacement reactions was outlined. A 10-bp spacer on AuNPs and fourfold amount of fuels were determined for good performance of this model with an optimized toehold strategy. After the reaction at 25 °C for 10 h, a 258 pM target could be identified, which is a remarkable improvement compared with the traditional AuNP–DNA system without fuel. Moreover, this model was also studied to differentiate specific single nucleotide polymorphism on target with superior selection factors. This model may help by introducing a proposition of target detection to guide further investigation.

中文翻译：

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金纳米颗粒对减少泄漏和信号放大中的催化DNA链位移的贡献

概述了使用金纳米颗粒（AuNP）-DNA系统限制渗漏并提高催化脚趾介导的链置换反应效率的新模型。确定了AuNPs上的10 bp间隔基和四倍量的燃料，以使该模型具有最佳的鞋头定位策略，从而具有良好的性能。在25°C下反应10小时后，可以确定258 pM的目标，与没有燃料的传统AuNP-DNA系统相比，这是一个显着的进步。此外，还研究了该模型以区分具有优良选择因子的靶标上的特定单核苷酸多态性。通过引入目标检测的命题来指导进一步的研究，该模型可能会有所帮助。