Highly selective probes hybridize only to fully complementary DNA or RNA sequences and, therefore, often fail to recognize mutated viral genomes. Here we designed a probe that possesses two seemingly incompatible properties: it tolerates some point mutations in genome, while it remains selective towards others. An OR deoxyribozyme logic gate was designed to fluorescently report the sequences of enterovirus 71 (EV71) covering ∼90% of all known EV71 strains. Importantly, sequences of closely related coxsackieviruses that differed by single nucleotides were reliably differentiated in 7 out of 8 cases.

中文翻译：

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高选择性检测病毒核酸的抗突变脱氧核酶或门

高度选择性的探针仅与完全互补的DNA或RNA序列杂交，因此通常无法识别突变的病毒基因组。在这里，我们设计了一种探针，该探针具有两个看似不兼容的特性：它可以耐受基因组中的某些点突变，而对其他突变仍然具有选择性。OR脱氧核酶逻辑门设计用于荧光报告覆盖所有已知EV71株约90％的肠病毒71（EV71）序列。重要的是，在8个病例中有7个可靠地区分了由单个核苷酸区别而来的紧密相关的柯萨奇病毒序列。