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Arabinosylation Modulates the Growth-Regulating Activity of the Peptide Hormone CLE40a from Soybean
Cell Chemical Biology ( IF 6.6 ) Pub Date : 2017-09-21 00:00:00 , DOI: 10.1016/j.chembiol.2017.08.014
Leo Corcilius 1 , April H Hastwell 2 , Mengbai Zhang 2 , James Williams 1 , Joel P Mackay 3 , Peter M Gresshoff 2 , Brett J Ferguson 2 , Richard J Payne 1
Affiliation  

Small post-translationally modified peptide hormones mediate crucial developmental and regulatory processes in plants. CLAVATA/ENDOSPERM-SURROUNDING REGION (CLE) genes are found throughout the plant kingdom and encode for 12–13 amino acid peptides that must often undergo post-translational proline hydroxylation and glycosylation withO-β1,2-triarabinose moieties before they become functional. Apart from a few recent examples, a detailed understanding of the structure and function of most CLE hormones is yet to be uncovered. This is mainly owing to difficulties in isolating mature homogeneously modified CLE peptides from natural plant sources. In this study, we describe the efficient synthesis of a synthetic Araf3Hyp glycosylamino acid building block that was used to access a hitherto uninvestigated CLE hormone from soybean called GmCLE40a. Through the development and implementation of a novelin vivoroot growth assay, we show that the synthetic triarabinosylated glycopeptide suppresses primary root growth in this important crop species.

中文翻译:


阿拉伯糖基化调节大豆肽激素 CLE40a 的生长调节活性



翻译后修饰的小肽激素介导植物的关键发育和调节过程。 CLAVATA/胚乳周围区域 (CLE) 基因遍布整个植物界,编码 12-13 个氨基酸肽,这些肽在发挥功能之前必须经常经历翻译后脯氨酸羟基化和 O-β1,2-三阿拉伯糖部分的糖基化。除了最近的几个例子外,对大多数 CLE 激素的结构和功能的详细了解尚未被揭示。这主要是由于从天然植物来源中分离成熟的均质修饰的 CLE 肽存在困难。在这项研究中,我们描述了合成 Araf3Hyp 糖基氨基酸结构单元的有效合成,该结构单元用于从大豆中获取迄今为止未经研究的 CLE 激素,称为 GmCLE40a。通过开发和实施新型体内根生长测定,我们表明合成的三阿拉伯糖基化糖肽抑制了这种重要作物物种的初生根生长。
更新日期:2017-09-21
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