A new near-infrared electrochemiluminescence resonance energy transfer (NECL-RET) strategy for enzyme-free amplified DNA detection was designed. In this strategy, the quaternary Cu-Zn-In-S nanocrystals (NCs) were applied as the ECL donor and gold nanostars (AuNSs) were used as the acceptor. The flowerlike MoS₂/GO/o-MWNTs nanostructure was synthesized and used as an outstanding substrate to immobilize the NCs. ECL measurements demonstrated that the NCs assembled on the MoS₂/GO/o-MWNTs film resulted in ECL intensity amplified by ~2.5-fold compared to that of the NCs assembled directly on the GCE. The Sem used here was not only as a coupling reagent to attach the NCs on the MoS₂/GO/o-MWNTs but also as a novel co-reaction accelerator to enhance the ECL intensity. In addition, we designed two hairpin DNA probes of H1 and H2, and based on the target-catalyzed hairpin assembly, tDNA could trigger the hybridization of the H1 and H2-AuNSs to be further released to initiate the next hybridization process to capture a larger number of H2-AuNSs on the electrode surface, which resulted in the quenching of the NCs ECL emission. Therefore, a dramatic increase in the ECL quenching efficiency proportional to the tDNA concentration was achieved. The linear range for tDNA detection was from 10 aM to 10 pM with a detection limit down to 10 aM. This design introduces the new concept of triple signal amplification into ECL determination, and it shows promise to be extended to provide a highly sensitive platform for various target DNA.

设计了一种新的近红外电化学发光共振能量转移（NECL-RET）策略，用于无酶扩增DNA检测。在该策略中，将四元Cu-Zn-In-S纳米晶体（NCs）用作ECL供体，将金纳米星（AuNSs）用作受体。合成了花状的MoS ₂ / GO / o-MWNTs纳米结构，并将其用作固定NC的出色底物。ECL测量表明，与直接在GCE上组装的NC相比，在MoS ₂ / GO / o-MWNTs薄膜上组装的NC的ECL强度放大了约2.5倍。此处使用的Sem不仅用作将NC附着在MoS ₂上的偶联剂/ GO / o-MWNTs还可以用作增强ECL强度的新型共反应促进剂。此外，我们设计了两个H1和H2发夹DNA探针，基于目标催化的发夹装配，tDNA可以触发H1和H2-AuNS的杂交进一步释放，从而启动下一个杂交过程以捕获更大的电极表面上的H2-AuNSs数量增加，导致NCs ECL发射淬灭。因此，实现了与tDNA浓度成正比的ECL猝灭效率的显着提高。tDNA检测的线性范围是10 aM至10 pM，检测限低至10 aM。该设计将三重信号放大的新概念引入到ECL测定中，并显示了有望扩展以为各种目标DNA提供高度敏感的平台的希望。