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Carrier assisted electromembrane extraction based on nonionic lipophilic surfactants for the determination of basic drugs in urine samples
Analytical Methods ( IF 2.7 ) Pub Date : 2017-08-16 00:00:00 , DOI: 10.1039/c7ay00673j Zolfaghar Aladaghlo 1, 2, 3, 4, 5 , Ali Reza Fakhari 1, 2, 3, 4, 5 , Kobra Sadat Hasheminasab 6, 7, 8, 9
Analytical Methods ( IF 2.7 ) Pub Date : 2017-08-16 00:00:00 , DOI: 10.1039/c7ay00673j Zolfaghar Aladaghlo 1, 2, 3, 4, 5 , Ali Reza Fakhari 1, 2, 3, 4, 5 , Kobra Sadat Hasheminasab 6, 7, 8, 9
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Herein, an efficient carrier assisted electromembrane extraction coupled with capillary electrophoresis has been proposed for the quantitation of basic drugs (pseudoephedrine and tramadol) in urine samples. Nonionic lipophilic surfactants in the supported liquid membrane act as carriers and promote the migration of ionic analytes toward the acceptor phase. The volumes of the sample and acceptor solutions were 4000 and 20 μL, respectively. All variables affecting the extraction efficiency were investigated for the proposed method. Optimal extractions were accomplished with 2-nitrophenyl octyl ether, containing 0.25 mM Span 80 as the supported liquid membrane, with 200 V as the driving force and with pH 5.0 in donor and pH 1 in acceptor solutions. Extractions were obtained after 30 min of operation with the whole assembly agitated at 1000 rpm. Under the optimum experimental conditions, the linear range was 10–2000 and 5–2000 ng mL−1 for pseudoephedrine and tramadol, respectively (R2 ≥ 0.99). The limits of detection were calculated to be 3.03 and 1.51 ng mL−1 for pseudoephedrine and tramadol, respectively. The relative standard deviation was lower than 4.1%. The enrichment factors were in the range of 178 to 188. The method was successfully applied for the analysis of these drugs in urine samples.
中文翻译：
基于非离子亲脂性表面活性剂的载体辅助电膜萃取法测定尿样中的碱性药物
在此，已经提出了一种有效的载体辅助的电膜提取与毛细管电泳相结合的方法，用于定量尿液样品中的基本药物（伪麻黄碱和曲马多）。负载的液膜中的非离子亲脂性表面活性剂充当载体，并促进离子分析物向受体相的迁移。样品溶液和受体溶液的体积分别为4000和20μL。研究了影响提取效率的所有变量。用2-硝基苯基辛醚进行最佳萃取，该醚含0.25 mM Span 80作为支撑液膜，驱动力为200 V，施主溶液的pH为5.0，受主溶液的pH为1。在操作30分钟后，将整个组件以1000 rpm的速度搅拌，获得提取物。-1用于伪麻黄碱和曲马多，分别为（ - [R 2 ≥0.99）。伪麻黄碱和曲马多的检出限分别为3.03和1.51 ng mL -1。相对标准偏差低于4.1％。富集因子在178至188范围内。该方法已成功用于尿液样品中这些药物的分析。
更新日期：2017-09-20
中文翻译：
基于非离子亲脂性表面活性剂的载体辅助电膜萃取法测定尿样中的碱性药物
在此，已经提出了一种有效的载体辅助的电膜提取与毛细管电泳相结合的方法，用于定量尿液样品中的基本药物（伪麻黄碱和曲马多）。负载的液膜中的非离子亲脂性表面活性剂充当载体，并促进离子分析物向受体相的迁移。样品溶液和受体溶液的体积分别为4000和20μL。研究了影响提取效率的所有变量。用2-硝基苯基辛醚进行最佳萃取，该醚含0.25 mM Span 80作为支撑液膜，驱动力为200 V，施主溶液的pH为5.0，受主溶液的pH为1。在操作30分钟后，将整个组件以1000 rpm的速度搅拌，获得提取物。-1用于伪麻黄碱和曲马多，分别为（ - [R 2 ≥0.99）。伪麻黄碱和曲马多的检出限分别为3.03和1.51 ng mL -1。相对标准偏差低于4.1％。富集因子在178至188范围内。该方法已成功用于尿液样品中这些药物的分析。
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