High throughput in vitro and in vivo screening of inland waters of Southern California,Environmental Science: Processes & Impacts

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High throughput in vitro and in vivo screening of inland waters of Southern California
Environmental Science: Processes & Impacts ( IF 4.3 ) Pub Date : 2017-06-14 00:00:00 , DOI: 10.1039/c7em00170c
A. C. Mehinto _{1,

2,

3} , D. R. VanDervort _{1,

2,

3} , W. Lao _{1,

2,

3} , G. He _{3,

4,

5} , M. S. Denison _{3,

4,

5} , S. M. Vliet _{3,

4,

6} , D. C. Volz _{3,

4,

6} , R. D. Mazor _{1,

2,

3} , K. A. Maruya _{1,

2,

3}

Affiliation

The impact of unmonitored contaminants, also known as contaminants of emerging concern (CECs), on freshwater streams remains largely uncharacterized. Water samples from 31 streams representing urban, agricultural and undeveloped (i.e., open space) land use in Southern California (USA) were analyzed for in vitro and in vivo bioactivity. The extent and magnitude of bioactivity screened using endocrine-responsive cell bioassays and a fish embryo screening assay were low. In contrast, a wider gradient of responses for the aryl hydrocarbon receptor (AhR) assay was observed, which was negatively correlated with a measure of benthic community structure. Both aromatic and non-aromatic CECs were tentatively identified in these samples, but polycyclic aromatic hydrocarbons (PAHs), known AhR agonists in urban environments, were not present at detectable levels. These results suggest that a combination of in vitro and in vivo show potential as screening techniques for biological condition in situ, but that more advanced, comprehensive analytical methods are needed to identify bioactive contaminants.

中文翻译：

高通量的南加州内陆水域体外和体内筛选

未经监控的污染物（也称为新兴关注的污染物（CEC））对淡水流的影响在很大程度上尚无定论。从31流代表城市，农业和欠发达（水样，即在南加州大学（美国），开放空间）的土地利用进行了分析体外和体内生物活性。使用内分泌反应性细胞生物测定法和鱼胚筛选测定法筛选的生物活性的程度和幅度较低。相比之下，芳烃受体（AhR）分析的响应梯度更大，与底栖生物群落结构的测量值呈负相关。这些样品初步鉴定出芳香族和非芳香族CEC，但城市环境中已知的AhR激动剂多环芳香烃（PAH）却未检出。这些结果表明体外和体内的组合显示出潜在的原位生物学条件筛选技术，但是需要更高级，更全面的分析方法来识别生物活性污染物。

更新日期：2017-06-14

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