The breadth of applications of nanoparticles and the access to food-associated consumer products containing nano-sized materials lead to oral human exposure to such particles. In biological fluids nanoparticles dynamically interact with biomolecules and form a protein corona. Knowledge about the protein corona is of great interest for understanding the molecular effects of particles as well as their fate inside the human body. We used a mass spectrometry-based toxicoproteomics approach to elucidate mechanisms of toxicity of silver nanoparticles and to comprehensively characterize the protein corona formed around silver nanoparticles in Caco-2 human intestinal epithelial cells. Results were compared with respect to the cellular function of proteins either affected by exposure to nanoparticles or present in the protein corona. A transcriptomic dataset was included in the analyses in order to obtain a combined multi-omics view of nanoparticle-affected cellular processes. A relationship between corona proteins and the proteomic or transcriptomic responses was revealed showing that differentially regulated proteins or transcripts were engaged in the same cellular signaling pathways. Protein corona analyses of nanoparticles in cells might therefore help obtaining information about the molecular consequences of nanoparticles treatment.

中文翻译：

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银纳米颗粒的蛋白质电晕分析与其细胞效应有关

纳米颗粒的广泛应用以及与含有纳米级材料的食品相关消费品的使用，导致人类口服这种颗粒。在生物流体中，纳米粒子与生物分子动态相互作用并形成蛋白质电晕。对蛋白质电晕的了解对于了解粒子在人体中的分子作用及其命运具有极大的兴趣。我们使用了基于质谱的毒理学方法，阐明了银纳米颗粒的毒性机理，并全面表征了在Caco-2人肠上皮细胞中银纳米颗粒周围形成的蛋白质电晕。比较了受纳米粒子影响或存在于蛋白质电晕中的蛋白质的细胞功能方面的结果。分析中包含转录组数据集，以便获得受纳米颗粒影响的细胞过程的组合多组学视图。揭示了电晕蛋白与蛋白质组或转录组反应之间的关系，表明差异调节的蛋白质或转录本参与相同的细胞信号传导途径。因此，对细胞中纳米颗粒的蛋白质电晕分析可能有助于获得有关纳米颗粒治疗的分子后果的信息。