A reliable and effective HPLC analytical method has been developed to stereoselectively quantify metconazole in soil and flour matrices. Effects of polysaccharide chiral stationary phase, type and content of alcoholic modifier on separation of racemic metconazole have been discussed in detail. Resolution and quantitative determination of metconazole stereoisomers were performed by using an Enantiopak OD column, with the n-hexane-ethanol mixture (97:3, v/v) at the flow rate of 1.0 mL/min. Then, extraction and cleanup procedures followed by the modified QuEChERS (quick, easy, cheap, effective, rugged and safe) method were used for metconazole racemate in soil and flour matrices. The residual analysis method was validated. Good linearity (R² ≥ 0.9997) and recoveries (94.98–104.89%, RSD ≤ 2.0%) for four metconazole stereoisomers were obtained. In brief, this proposed method showed good accuracy and precision, which might be applied in enantioselective determination, residual quantitative analysis, and degradation of metconazole in food and environmental matrices.

已经开发出一种可靠有效的HPLC分析方法，可对土壤和面粉基质中的甲康唑进行立体选择性定量分析。详细讨论了多糖手性固定相，醇改性剂的类型和含量对外消旋甲康唑分离的影响。使用Enantiopak OD色谱柱和正己烷-乙醇混合物（97：3，v / v）以1.0 mL / min的流速进行拆分和定量测定甲康唑立体异构体。然后，采用萃取和净化程序，然后采用改良的QuEChERS（快速，简便，廉价，有效，坚固和安全）方法处理土壤和面粉基质中的甲康唑消旋体。残差分析方法得到验证。良好的线性度（R ²≥0.9997）并获得了四种甲康唑立体异构体的回收率（94.98–104.89％，RSD≤2.0％）。简而言之，该方法具有良好的准确度和精密度，可用于对映选择性测定，残留定量分析以及食品和环境基质中甲康唑的降解。