Analysis of deferiprone in exhaled breath condensate using silver nanoparticle-enhanced terbium fluorescence,Analytical Methods

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Analysis of deferiprone in exhaled breath condensate using silver nanoparticle-enhanced terbium fluorescence
Analytical Methods ( IF 2.7 ) Pub Date : 2017-09-19 00:00:00 , DOI: 10.1039/c7ay01715d
Esmail Mohamadian _{1,

2,

3,

4} , Ali Shayanfar _{2,

3,

4,

5} , Maryam Khoubnasabjafari _{2,

3,

4,

6} , Vahid Jouyban-Gharamaleki _{2,

3,

4,

7,

8} , Saba Ghaffary _{2,

4,

9,

10} , Abolghasem Jouyban _{2,

3,

4,

5,

8}

Affiliation

Exhaled breath condensate (EBC) has been proposed as an alternative non-invasive biological sample for therapeutic drug monitoring. In this study, a sensitive method for the quantification of deferiprone (DEF) in EBC samples was developed using a silver nanoparticle (AgNP)-enhanced terbium (Tb³⁺) fluorescence method. Fluorescence properties of the Tb³⁺–DEF complex were studied in the presence of AgNPs in EBC. Effects of various parameters such as AgNPs and Tb³⁺ concentrations, pH, and buffer concentration were studied. A calibration curve was obtained with a coefficient of determination (R²) of 0.999, and linearity was observed in the concentration range of 0.06–1.50 mg L⁻¹. The method was successfully applied for the quantification of DEF in sixteen patients with very low concentrations (0.06–0.17 mg L⁻¹) in the EBC samples.

中文翻译：

纳米银增强ter荧光分析法分析呼出气冷凝物中的去铁酮

已提出呼出气冷凝物（EBC）作为用于治疗性药物监测的替代性非侵入性生物样品。在这项研究中，使用银纳米颗粒（AgNP）增强的（（Tb ³⁺）荧光方法开发了一种定量EBC样品中去铁酮（DEF）的灵敏方法。在EBC中存在AgNPs的情况下研究了Tb ³⁺ -DEF复合物的荧光性质。研究了各种参数（例如AgNPs和Tb ³⁺浓度，pH和缓冲液浓度）的影响。获得的测定曲线（R ²）为0.999，在0.06-1.50 mg L ^-1的浓度范围内观察到线性。该方法已成功应用于16例EBC样品中极低浓度（0.06-0.17 mg L ^-1）患者的DEF定量。

更新日期：2017-09-19

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