Double-limitation systems have shown to induce polyhydroxyalkanoates (PHA) production in chemostat studies limited in e.g. carbon and phosphate. In this work the impact of double substrate limitation on the enrichment of a PHA producing community was studied in a sequencing batch process. Enrichments at different C/P concentration ratios in the influent were established and the effect on the PHA production capacity and the enrichment community structure was investigated. Experimental results demonstrated that when a double substrate limitation is imposed at a C/P ratio in the influent in a range of 150 (C-mol/mol), the P-content of the biomass and the specific substrate uptake rates decreased. Nonetheless, the PHA storage capacity remained high (with a maximum of 84 wt%). At a C/P ratio of 300, competition in the microbial community is based on phosphate uptake, and the PHA production capacity is lost. Biomass specific substrate uptake rates are a linear function of the cellular P-content, offering advantages for scaling-up the PHA production process due to lower oxygen requirements.

中文翻译：

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磷的限制对饥荒过程中PHA产生的影响。

在限制于例如碳和磷酸盐的化学恒温器研究中，双限制系统已显示出可诱导多羟基链烷酸酯（PHA）的产生。在这项工作中，在测序分批过程中研究了双重底物限制对PHA产生群落富集的影响。建立了进水中不同C / P浓度比的富集，并研究了其对PHA生产能力和富集群落结构的影响。实验结果表明，当进水的C / P比在150（C-mol / mol）范围内施加双重底物限制时，生物质的P含量和特定底物吸收率会降低。尽管如此，PHA的存储容量仍然很高（最大为84 wt％）。在C / P为300的情况下，微生物界的竞争是基于磷酸盐的吸收，PHA的生产能力已经丧失。生物质特定底物的吸收速率是细胞P含量的线性函数，由于较低的氧气需求，为扩大PHA生产工艺提供了优势。