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A mitochondria-targeted fluorescent probe based on fluorescein derivative for detection of hypochlorite in living cells
Dyes and Pigments ( IF 4.1 ) Pub Date : 2017-09-18 , DOI: 10.1016/j.dyepig.2017.09.037
Jing Lv , Yahui Chen , Fang Wang , Tingwen Wei , Zhijie Zhang , Jian Qiang , Xiaoqiang Chen

In the present study, a mitochondria-targeted fluorescent probe FL based on fluorescein derivative for hypochlorite sensing was designed and synthesized. The probe FL showed highly selective to HOCl among various reactive oxygen/nitrogen species. With the increasing amount of HOCl from 0 to 50 μM, a linear correlation between the fluorescence intensity of FL at 530 nm and the concentration of HOCl was found. The detection limit is calculated to be 0.068 μM. Furthermore, the probe demonstrated a perfect mitochondria targetable ability and it was successfully applied to image mitochondrial HOCl in living HeLa cells. The success of subcellular imaging indicated that the mitochondria-targetable probe FL might be further used to investigate biological functions and pathological roles of HOCl at organelle levels.



中文翻译:

基于荧光素衍生物的线粒体靶向荧光探针可检测活细胞中的次氯酸盐

In the present study, a mitochondria-targeted fluorescent probe FL based on fluorescein derivative for hypochlorite sensing was designed and synthesized. The probe FL showed highly selective to HOCl among various reactive oxygen/nitrogen species. With the increasing amount of HOCl from 0 to 50 μM, a linear correlation between the fluorescence intensity of FL at 530 nm and the concentration of HOCl was found. The detection limit is calculated to be 0.068 μM. Furthermore, the probe demonstrated a perfect mitochondria targetable ability and it was successfully applied to image mitochondrial HOCl in living HeLa cells. The success of subcellular imaging indicated that the mitochondria-targetable probe FL 可能会进一步用于研究细胞器水平上HOCl的生物学功能和病理作用。

更新日期:2017-09-18
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