The first multi-channel probe giving different signals towards ROS and nitroxyl has been easily synthesized with a rational method which successfully showed blue emission towards peroxynitrite and hypochlorite and green emission towards nitroxyl in solution. The highly efficient oxidation of hypochlorite towards triphenylphosphine was discovered and utilized to distinguish hypochlorite and peroxynitrite with the aid of nitroxyl. A rare photoinduced electron transfer (PET) phenomenon from the donor fluorophore to the acceptor fluorophore was observed and adopted to give the signal of sequence sensing. Furthermore, the probe gave real-time signal selectively towards peroxynitrite and nitroxyl in Hela cells.

使用合理的方法可以轻松合成第一个向ROS和硝氧基发出不同信号的多通道探针，该方法成功地显示了溶液中过氧亚硝酸盐和次氯酸盐的蓝光发射以及硝酸盐的绿光发射。发现了次氯酸盐向三苯基膦的高效氧化，并在硝氧基的帮助下用于区分次氯酸盐和过氧亚硝酸盐。观察到罕见的从供体荧光团到受体荧光团的光致电子转移（PET）现象，并被用于提供序列感测信号。此外，该探针可选择性地向Hela细胞中的过氧亚硝酸盐和亚硝酰基提供实时信号。