Determination of Quercetin and Luteolin in Paprika Samples by Voltammetry and Partial Least Squares Calibration,Electroanalysis

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Determination of Quercetin and Luteolin in Paprika Samples by Voltammetry and Partial Least Squares Calibration
Electroanalysis ( IF 2.7 ) Pub Date : 2017-09-19 , DOI: 10.1002/elan.201700403
Francisco Chamizo-González ₁ , Olga Monago-Maraña _{1,

2} , Teresa Galeano-Díaz _{1,

2}

Affiliation

Quercetin and luteolin are flavonoids with beneficious properties, which are present in paprika. In this work, both have been determined in paprika by using electrochemistry combined with chemometrics. The electrochemical oxidation mechanisms of both analytes have been studied through sampled direct current (DC) voltammetry, differential pulse voltammetry (DPV) and Square Wave Voltammetry (SWV), making use of a glassy carbon electrode. The final technique selected for the quantification was DPV due to its high repeatability with respect SWV. The chemical variables and the instrumental parameters were optimized and the final conditions employed were ethanol: water (20 : 80), 0.75 mol dm−3 of HCl, and a pulse amplitude of 50 mV. Due to the facts that oxidation potential of both analytes were quite similar, their DPV peaks were overlapped, and also because the analytes interaction during the electrochemical process causes a non-additivity of the signals, they could not be quantified separately by direct measurement of peak intensity. For this reason, a chemometric algorithm was applied (partial least squares (PLS) regression in its modality PLS-2). In the case of validation samples, appropriate sets of calibration and validation were built and good results were obtained. This methodology was applied to real paprika samples and the results were similar to those obtained with a HPLC method previously reported.

中文翻译：

伏安法和偏最小二乘校准法测定辣椒粉样品中的槲皮素和木犀草素

槲皮素和木犀草素是具有有益特性的黄酮类化合物，它们存在于辣椒粉中。在这项工作中，通过使用电化学结合化学计量学在辣椒粉中测定了两者。已经通过采样直流 (DC) 伏安法、差分脉冲伏安法 (DPV) 和方波伏安法 (SWV) 研究了两种分析物的电化学氧化机制，利用玻碳电极。选择用于量化的最终技术是 DPV，因为它在 SWV 方面具有高重复性。优化了化学变量和仪器参数，最终采用的条件是乙醇：水（20：80）、0.75 mol dm-3 的 HCl 和 50 mV 的脉冲幅度。由于两种分析物的氧化电位非常相似，因此它们的 DPV 峰重叠，此外，由于电化学过程中分析物的相互作用导致信号的非可加性，因此无法通过直接测量峰强度来单独量化它们。为此，应用了化学计量算法（偏最小二乘 (PLS) 回归在其模态 PLS-2 中）。在验证样本的情况下，建立了适当的校准和验证集，并获得了良好的结果。该方法应用于真实的辣椒粉样品，结果与之前报道的 HPLC 方法获得的结果相似。应用了化学计量算法（偏最小二乘 (PLS) 回归在其模态 PLS-2 中）。在验证样本的情况下，建立了适当的校准和验证集，并获得了良好的结果。该方法应用于真实的辣椒粉样品，结果与之前报道的 HPLC 方法获得的结果相似。应用了化学计量算法（偏最小二乘 (PLS) 回归在其模态 PLS-2 中）。在验证样本的情况下，建立了适当的校准和验证集，并获得了良好的结果。该方法应用于真实的辣椒粉样品，结果与之前报道的 HPLC 方法获得的结果相似。

更新日期：2017-09-19

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