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High-Flow-Rate Impinger for the Study of Concentration, Viability, Metabolic Activity, and Ice-Nucleation Activity of Airborne Bacteria
Environmental Science & Technology ( IF 10.8 ) Pub Date : 2017-09-19 00:00:00 , DOI: 10.1021/acs.est.7b01480 Tina Šantl-Temkiv 1, 2, 3 , Pierre Amato 4 , Ulrich Gosewinkel 5 , Runar Thyrhaug 6 , Anaïs Charton 4 , Benjamin Chicot 4 , Kai Finster 2, 3 , Gunnar Bratbak 6 , Jakob Löndahl 1
Environmental Science & Technology ( IF 10.8 ) Pub Date : 2017-09-19 00:00:00 , DOI: 10.1021/acs.est.7b01480 Tina Šantl-Temkiv 1, 2, 3 , Pierre Amato 4 , Ulrich Gosewinkel 5 , Runar Thyrhaug 6 , Anaïs Charton 4 , Benjamin Chicot 4 , Kai Finster 2, 3 , Gunnar Bratbak 6 , Jakob Löndahl 1
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The study of airborne bacteria relies on a sampling strategy that preserves their integrity and in situ physiological state, e.g. viability, cultivability, metabolic activity, and ice-nucleation activity. Because ambient air harbors low concentrations of bacteria, an effective bioaerosol sampler should have a high sampling efficiency and a high airflow. We characterize a high-flow-rate impinger with respect to particle collection and retention efficiencies in the range 0.5–3.0 μm, and we investigated its ability to preserve the physiological state of selected bacterial species and seawater bacterial community in comparison with four commercial bioaerosol samplers. The collection efficiency increased with particle size and the cutoff diameter was between 0.5 and 1 μm. During sampling periods of 120–300 min, the impinger retained the cultivability, metabolic activity, viability, and ice-nucleation activity of investigated bacteria. Field studies in semiurban, high-altitude, and polar environments included periods of low bacterial air concentrations, thus demonstrating the benefits of the impinger’s high flow rate. In conclusion, the impinger described here has many advantages compared with other bioaerosol samplers currently on the market: a potential for long sampling time, a high flow rate, a high sampling and retention efficiency, low costs, and applicability for diverse downstream microbiological and molecular analyses.
中文翻译：
高流速撞击器，用于研究机载细菌的浓度，生存力，代谢活性和冰核活性
空气传播细菌的研究依赖于保留其完整性和原位生理状态（例如生存力，可培养性，代谢活性和冰核活性）的采样策略。由于周围空气中细菌含量低，因此有效的生物气溶胶采样器应具有较高的采样效率和较高的气流。我们针对颗粒收集和保留效率在0.5–3.0μm范围内的高流速撞击器进行了表征，并与四个商业生物气溶胶采样器相比，我们研究了其保留选定细菌物种和海水细菌群落的生理状态的能力。收集效率随粒度而增加，并且截止直径在0.5和1μm之间。在120-300分钟的采样期间，冲击器保持了可耕性，被调查细菌的代谢活性，生存力和冰核活性。在半城市，高海拔和极地环境中的现场研究包括细菌空气浓度低的时期，从而证明了冲击器高流速的好处。总之，与目前市场上的其他生物气溶胶采样器相比，此处描述的冲击器具有许多优点：采样时间长，流速高，采样和保留效率高，成本低以及可用于各种下游微生物和分子的潜力分析。
更新日期：2017-09-19
中文翻译：
高流速撞击器，用于研究机载细菌的浓度，生存力，代谢活性和冰核活性
空气传播细菌的研究依赖于保留其完整性和原位生理状态（例如生存力，可培养性，代谢活性和冰核活性）的采样策略。由于周围空气中细菌含量低，因此有效的生物气溶胶采样器应具有较高的采样效率和较高的气流。我们针对颗粒收集和保留效率在0.5–3.0μm范围内的高流速撞击器进行了表征，并与四个商业生物气溶胶采样器相比，我们研究了其保留选定细菌物种和海水细菌群落的生理状态的能力。收集效率随粒度而增加，并且截止直径在0.5和1μm之间。在120-300分钟的采样期间，冲击器保持了可耕性，被调查细菌的代谢活性，生存力和冰核活性。在半城市，高海拔和极地环境中的现场研究包括细菌空气浓度低的时期，从而证明了冲击器高流速的好处。总之，与目前市场上的其他生物气溶胶采样器相比，此处描述的冲击器具有许多优点：采样时间长，流速高，采样和保留效率高，成本低以及可用于各种下游微生物和分子的潜力分析。
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