Nanofibrillated bacterial cellulose (NFBC) is produced by culturing a cellulose-producing bacterium (Gluconacetobacter intermedius NEDO-01) with rotation or agitation in medium supplemented with carboxymethylcellulose (CMC). Despite a high yield and dispersibility in water, the product immediately aggregates in organic solvents. To broaden its applicability, we prepared amphiphilic NFBC by culturing strain NEDO-01 in medium supplemented with hydroxyethylcellulose or hydroxypropylcellulose instead of CMC. Transmission electron microscopy analysis revealed that the resultant materials (HE-NFBC and HP-NFBC, respectively) comprised relatively uniform fibers with diameters of 33 ± 7 and 42 ± 8 nm, respectively. HP-NFBC was dispersible in polar organic solvents such as methanol, acetone, isopropyl alcohol, acetonitrile, tetrahydrofuran (THF), and dimethylformamide, and was also dispersible in poly(methyl methacrylate) (PMMA) by solvent mixing using THF. HP-NFBC/PMMA composite films were highly transparent and had a higher tensile strength than neat PMMA film. Thus, HP-NFBC has a broad range of applications, including as a filler material.

中文翻译：

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使用产生纤维素的细菌一步生产两亲性纳米原纤化纤维素

纳纤化细菌纤维素（NFBC）是通过培养产生纤维素的细菌（中间糖杆菌）生产的NEDO-01）在补充有羧甲基纤维素（CMC）的培养基中旋转或搅拌。尽管产量高且在水中分散性高，但该产品立即在有机溶剂中聚集。为了扩大其适用性，我们通过在补充有羟乙基纤维素或羟丙基纤维素而不是CMC的培养基中培养菌株NEDO-01来制备两亲性NFBC。透射电子显微镜分析显示，所得材料（分别为HE-NFBC和HP-NFBC）包含直径分别为33±7和42±8nm的相对均匀的纤维。HP-NFBC可分散在极性有机溶剂（例如甲醇，丙酮，异丙醇，乙腈，四氢呋喃（THF）和二甲基甲酰胺）中，也可通过使用THF混合溶剂分散在聚（甲基丙烯酸甲酯）（PMMA）中。与纯PMMA膜相比，HP-NFBC / PMMA复合膜具有很高的透明度，并具有更高的拉伸强度。因此，HP-NFBC具有广泛的应用，包括作为填充材料。