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Ultrasensitive MicroRNA Assay via Surface Plasmon Resonance Responses of Au@Ag Nanorods Etching
Analytical Chemistry ( IF 6.7 ) Pub Date : 2017-09-19 00:00:00 , DOI: 10.1021/acs.analchem.7b02920 Yu Gu 1 , Juan Song 1 , Mei-Xing Li 1 , Ting-Ting Zhang 1 , Wei Zhao 1 , Jing-Juan Xu 1 , Maili Liu 2 , Hong-Yuan Chen 1
Analytical Chemistry ( IF 6.7 ) Pub Date : 2017-09-19 00:00:00 , DOI: 10.1021/acs.analchem.7b02920 Yu Gu 1 , Juan Song 1 , Mei-Xing Li 1 , Ting-Ting Zhang 1 , Wei Zhao 1 , Jing-Juan Xu 1 , Maili Liu 2 , Hong-Yuan Chen 1
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Quantification of trace serum circulate microRNAs is extremely important in clinical diagnosis but remains a great challenge. Herein we developed an ultrasensitive platform for microRNA 141 (miR-141) detection based on a silver coated gold nanorods (Au@Ag NRs) etching process accompanied by surface plasmon resonance (SPR) shift. Both SPR absorption and scattering responses were monitored. Combined amplification cascades of catalyzed hairpin assembly (CHA) and hybridization chain reaction (HCR) with the sensitive SPR responses of plasmonic Au@Ag NRs, the proposed bioassay exhibited ultrahigh sensitivity toward miRNA-141 with dynamic range from 5.0 × 10–17 M to 1.0 × 10–11 M. With target concentration higher than 1.0 × 10–13 M, the color of the solution changed obviously that could be observed with naked eyes. Under dark-field microscopy observation of individual particle, a limit of detection down to 50 aM could be achieved. Owing to the superior sensitivity and selectivity, the proposed method was applied to detecting trace microRNA in serum. Similar SPR assays could be developed simply by redesigning the switching aptamer for the detections of other microRNAs or targets such as small molecule, DNA, or protein. Considering the convenient operation, good performance and simple observation modes of this method, it may have great potential in trace bioanalysis for clinical applications.
中文翻译：
通过Au @ Ag纳米棒刻蚀的表面等离子体共振响应进行超灵敏MicroRNA分析
微量血清循环微RNA的定量在临床诊断中非常重要，但仍然是一个巨大的挑战。本文中，我们开发了一种超敏感平台，用于基于涂银金纳米棒（Au @ Ag NRs）蚀刻过程并伴随表面等离振子共振（SPR）移位的microRNA 141（miR-141）检测。监测SPR吸收和散射响应。结合催化的发夹装配（CHA）和杂交链反应（HCR）的扩增级联反应与等离子体Au @ Ag NRs的敏感SPR反应，拟议的生物测定对miRNA-141表现出超高灵敏度，动态范围为5.0×10 –17 M 1.0×10 –11M。目标浓度高于1.0×10 –13M，溶液的颜色明显改变，可以用肉眼观察到。在暗视野显微镜下观察单个颗粒，可以实现低至50 aM的检测限。由于其优越的灵敏度和选择性，该方法被用于检测血清中的微量microRNA。通过重新设计用于检测其他microRNA或靶标（例如小分子，DNA或蛋白质）的转换适体，可以简单地开发出类似的SPR分析法。考虑到该方法操作简便，性能好，观察方式简单等优点，在痕量生物分析中具有广阔的应用前景。
更新日期：2017-09-19
中文翻译：
通过Au @ Ag纳米棒刻蚀的表面等离子体共振响应进行超灵敏MicroRNA分析
微量血清循环微RNA的定量在临床诊断中非常重要，但仍然是一个巨大的挑战。本文中，我们开发了一种超敏感平台，用于基于涂银金纳米棒（Au @ Ag NRs）蚀刻过程并伴随表面等离振子共振（SPR）移位的microRNA 141（miR-141）检测。监测SPR吸收和散射响应。结合催化的发夹装配（CHA）和杂交链反应（HCR）的扩增级联反应与等离子体Au @ Ag NRs的敏感SPR反应，拟议的生物测定对miRNA-141表现出超高灵敏度，动态范围为5.0×10 –17 M 1.0×10 –11M。目标浓度高于1.0×10 –13M，溶液的颜色明显改变，可以用肉眼观察到。在暗视野显微镜下观察单个颗粒，可以实现低至50 aM的检测限。由于其优越的灵敏度和选择性，该方法被用于检测血清中的微量microRNA。通过重新设计用于检测其他microRNA或靶标（例如小分子，DNA或蛋白质）的转换适体，可以简单地开发出类似的SPR分析法。考虑到该方法操作简便，性能好，观察方式简单等优点，在痕量生物分析中具有广阔的应用前景。
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