Construction of Smart Glutathione S-Transferase via Remote Optically Controlled Supramolecular Switches,ACS Catalysis

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Construction of Smart Glutathione S-Transferase via Remote Optically Controlled Supramolecular Switches
ACS Catalysis ( IF 11.3 ) Pub Date : 2017-09-19 00:00:00 , DOI: 10.1021/acscatal.7b02821
Yao Liu ₁ , Tiezheng Pan _{1,

2} , Yu Fang ₁ , Ningning Ma ₁ , Shanpeng Qiao ₁ , Linlu Zhao ₁ , Ruidi Wang ₁ , Tingting Wang ₁ , Xiumei Li ₁ , Xiaojia Jiang ₁ , Fangzhong Shen ₁ , Quan Luo ₁ , Junqiu Liu ₁

Affiliation

A supramolecular switch strategy that can reversibly “turn-on” and “turn-off” glutathione S-transferase (GST) is presented, which provides a proof-of-concept for a simple but efficient way to regulate the catalytic function of natural enzymes. This design is demonstrated by incorporating azobenzene/cyclodextrin-based supramolecular host–guest systems into the catalytic pocket of GST. The photoisomerization of trans- and cis-azobenzene leads to supramolecular complexation and dissociation of cyclodextrin, and thereby controls the enzymatic activity of GST by tuning substrate accessibility. This photoswitchable catalysis is reversible over multiple stimulus cycles. Furthermore, its capability is affected by the spatial size and binding affinity of different cyclodextrins, as well as the modification sites of azobenzene. The remote optical modulation method could offer great opportunities in the effort to create “smart” catalysts.

中文翻译：

远程光控超分子开关构建智能型谷胱甘肽S-转移酶

提出了一种超分子转换策略，该策略可以可逆地“开启”和“关闭”谷胱甘肽S-转移酶（GST），为简单而有效的调节天然酶催化功能的方法提供了概念验证。通过将基于偶氮苯/环糊精的超分子主客体系统整合到GST的催化口袋中，可以证明这种设计。的光致异构反式-与顺-偶氮苯导致超环糊精和环糊精的解离，从而通过调节底物的可及性来控制GST的酶活性。这种光开关催化在多个刺激循环中是可逆的。此外，其能力受不同环糊精的空间大小和结合亲和力以及偶氮苯的修饰位点影响。远程光调制方法可以为制造“智能”催化剂提供巨大的机会。

更新日期：2017-09-19

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