In this research, the manual two-step QuEChERS approach has been streamlined and automated into a one-step method using a cleanup tube fitted within an extraction tube. A novel automatic QuEChERS combination have been developed to simplify the QuEChERS procedures and improve sample preparation efficiency. This combination integrates QuEChERS procedures into a single run via the use of a vortex vibration-centrifuge device and a centrifuge filtration tube. To validate the efficiency of our automatic QuEChERS device, 270 pesticides were analyzed in plant origined foods including celery, tomatoes, leeks, eggplants, grapes, corn, green tea, and soybean oil using this automatic platform. The results were then compared with those obtained using the manual QuEChERS method. Different parameters were validated and compared including recovery, linearity, repeatability and limits of quantification (LOQ). Satisfactory results, comparable to results obtained using the manual QuEChERS method were obtained. The average recoveries ranged between 70% and 120% for most pesticides with associated relative standard deviations (RSDs) <20% (n = 5) indicating satisfactory accuracy and repeatability. An LOQ of 2 μg/kg was obtained for most pesticides present in celery and corn matrices, and the correlation coefficients (r²) were >0.990 within a linearity range of 2–500 μg/kg. Compared to manual QuEChERS, this novel automatic QuEChERS device and combination could significantly improve the sample preparation efficiency for the multiresidue analysis of pesticides.

在这项研究中，手动两步QuEChERS方法已经简化，并使用安装在提取管中的净化管自动转换为一步法。已开发出新颖的自动QuEChERS组合，以简化QuEChERS程序并提高样品制备效率。这种组合集成的QuEChERS程序到一个单一的运行通过使用涡旋振动离心装置和离心过滤管。为了验证我们自动QuEChERS设备的效率，使用该自动平台对植物来源的食品（包括芹菜，西红柿，韭菜，茄子，葡萄，玉米，绿茶和豆油）中的270种农药进行了分析。然后将结果与使用手动QuEChERS方法获得的结果进行比较。验证并比较了不同的参数，包括回收率，线性，重复性和定量限（LOQ）。获得了令人满意的结果，与使用手动QuEChERS方法获得的结果相当。大多数农药的平均回收率在70％到120％之间，相关的相对标准偏差（RSD）<20％（n = 5），表明令人满意的准确性和可重复性。²）在2–500μg/ kg的线性范围内> 0.990。与手动QuEChERS相比，这种新颖的自动QuEChERS设备及其组合可以显着提高用于农药多残留分析的样品制备效率。