Untargeted GC-TOF-MS analysis proved to be a suitable analytical platform to determine alterations in the metabolic profile. Several metabolic pathways were found to be altered in a first clinical study comparing smokers against nonsmokers. Subsequently, we conducted a clinical diet-controlled study to investigate alterations in the metabolic profile during the course of 3 months of smoking cessation. Sixty male subjects were included in the study, and plasma, saliva, and urine samples were collected during four 24 h stationary visits: at baseline, while still smoking, after 1 week, after 1 month, and after 3 months of cessation. Additionally, subjects were monitored for their compliance by measurements of CO in exhaled breath and salivary cotinine throughout the study. GC-TOF-MS fingerprinting was applied to plasma, saliva, and urine samples derived from 39 compliant subjects. In total, 52 metabolites were found to be significantly altered including 26 in plasma, 20 in saliva, and 12 in urine, respectively. In agreement with a previous study comparing smokers and nonsmokers, the fatty acid and amino acid metabolism showed significant alterations upon 3 months of smoking cessation. Thus these results may indicate a partial recovery of metabolic pathway perturbations, even after a relatively short period of smoking cessation.

中文翻译：

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使用经验证的GC-TOF-MS代谢组学平台进行饮食控制的临床戒烟研究中各种体液的代谢组学指纹图谱

无目标GC-TOF-MS分析被证明是确定代谢谱变化的合适分析平台。在比较吸烟者与不吸烟者的第一项临床研究中，发现了几种代谢途径被改变。随后，我们进行了一项临床饮食控制研究，以研究戒烟3个月期间新陈代谢特征的变化。这项研究包括60名男性受试者，并在四个24小时的固定就诊期间收集了血浆，唾液和尿液样本：在基线，仍吸烟的情况下，在1周后，1个月后和戒烟3个月后。另外，在整个研究过程中，通过测量呼出气和唾液可替宁中的CO来监测受试者的依从性。GC-TOF-MS指纹图谱用于血浆，唾液，以及来自39位依从对象的尿液样本。总共发现52种代谢物发生了显着变化，包括血浆中的26种，唾液中的20种和尿液中的12种。与先前比较吸烟者和非吸烟者的研究一致，在戒烟3个月后，脂肪酸和氨基酸代谢显示出显着变化。因此，即使在相对较短的戒烟时间后，这些结果也可能表明代谢途径扰动得以部分恢复。