Clickable Multifunctional Dumbbell Particles for in Situ Multiplex Single-Cell Cytokine Detection,ACS Applied Materials & Interfaces

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Clickable Multifunctional Dumbbell Particles for in Situ Multiplex Single-Cell Cytokine Detection
ACS Applied Materials & Interfaces ( IF 8.3 ) Pub Date : 2017-09-18 00:00:00 , DOI: 10.1021/acsami.7b08338
Peng Zhao ₁ , Justin George ₁ , Bin Li ₁ , Nooshin Amini ₂ , Janet Paluh ₂ , Jun Wang _{1,

3}

Affiliation

We report a novel strategy for fabrication of multifunctional dumbbell particles (DPs) through click chemistry for monitoring single-cell cytokine releasing. Two different types of DPs were prepared on a large scale through covalent bioorthogonal reaction between methyltetrazine and trans-cyclooctene on a microchip under a magnetic field. After collection of the DPs, the two sides of each particle were further functionalized with different antibodies for cell capturing and cytokine detection, respectively. These DPs labeled with different fluorescent dyes have been used for multiplex detection and analysis of cytokines secreted by single live cells. Our results show that this new type of DPs are promising for applications in cell sorting, bioimaging, single-cell analysis, and biomedical diagnostics.

中文翻译：

可单击的多功能哑铃粒子，用于原位多重单细胞细胞因子检测

我们报告了通过点击化学监测单细胞细胞因子释放制造多功能哑铃颗粒（DPs）的新策略。通过在磁场下微芯片上甲基四嗪与反式环辛烯之间的共价生物正交反应，大规模制备了两种不同类型的DP。收集DP后，分别用不同的抗体对每个颗粒的两侧进行功能化，分别用于细胞捕获和细胞因子检测。这些用不同荧光染料标记的DP已用于多重检测和分析单个活细胞分泌的细胞因子。我们的结果表明，这种新型的DP有望用于细胞分选，生物成像，单细胞分析和生物医学诊断。

更新日期：2017-09-19

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