Terminating DNA Tile Assembly with Nanostructured Caps,ACS Nano

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Terminating DNA Tile Assembly with Nanostructured Caps
ACS Nano ( IF 15.8 ) Pub Date : 2017-09-18 00:00:00 , DOI: 10.1021/acsnano.7b02256
Deepak K. Agrawal ₁ , Ruoyu Jiang ₁ , Seth Reinhart ₁ , Abdul M. Mohammed ₁ , Tyler D. Jorgenson ₁ , Rebecca Schulman _{1,

2}

Affiliation

Precise control over the nucleation, growth, and termination of self-assembly processes is a fundamental tool for controlling product yield and assembly dynamics. Mechanisms for altering these processes programmatically could allow the use of simple components to self-assemble complex final products or to design processes allowing for dynamic assembly or reconfiguration. Here we use DNA tile self-assembly to develop general design principles for building complexes that can bind to a growing biomolecular assembly and terminate its growth by systematically characterizing how different DNA origami nanostructures interact with the growing ends of DNA tile nanotubes. We find that nanostructures that present binding interfaces for all of the binding sites on a growing facet can bind selectively to growing ends and stop growth when these interfaces are presented on either a rigid or floppy scaffold. In contrast, nucleation of nanotubes requires the presentation of binding sites in an arrangement that matches the shape of the structure’s facet. As a result, it is possible to build nanostructures that can terminate the growth of existing nanotubes but cannot nucleate a new structure. The resulting design principles for constructing structures that direct nucleation and termination of the growth of one-dimensional nanostructures can also serve as a starting point for programmatically directing two- and three-dimensional crystallization processes using nanostructure design.

中文翻译：

终止带有纳米结构帽的DNA瓷砖组装

精确控制自组装过程的成核，生长和终止是控制产品良率和组装动态的基本工具。用于以编程方式更改这些过程的机制可以允许使用简单的组件自组装复杂的最终产品，或者设计允许动态组装或重新配置的过程。在这里，我们使用DNA瓦片自组装来开发通用的设计原理，以构建可以与不断增长的生物分子组装体结合并通过系统表征不同的DNA折纸纳米结构与DNA瓦片纳米管的生长端相互作用的复合物终止其生长的复合物。我们发现，当这些界面出现在刚性或软盘支架上时，呈现出生长小面上所有结合位点的结合界面的纳米结构可以选择性地结合到生长的末端并停止生长。相反，纳米管的成核要求以与结构的小平面形状相匹配的排列方式呈现结合位点。结果，可以构建可以终止现有纳米管的生长但不能成核新结构的纳米结构。用于构造指导一维纳米结构的成核和终止生长的结构的设计原理也可以用作使用纳米结构设计以程序方式指导二维和三维结晶过程的起点。

更新日期：2017-09-19

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