The adsorption of serum proteins, leading to the formation of a biomolecular corona, is a key determinant of the biological identity of nanoparticles in vivo. Therefore, gaining knowledge on the formation, composition, and temporal evolution of the corona is of utmost importance for the development of nanoparticle‐based therapies. Here, it is shown that the use of super‐resolution optical microscopy enables the imaging of the protein corona on mesoporous silica nanoparticles with single protein sensitivity. Particle‐by‐particle quantification reveals a significant heterogeneity in protein absorption under native conditions. Moreover, the diversity of the corona evolves over time depending on the surface chemistry and degradability of the particles. This paper investigates the consequences of protein adsorption for specific cell targeting by antibody‐functionalized nanoparticles providing a detailed understanding of corona‐activity relations. The methodology is widely applicable to a variety of nanostructures and complements the existing ensemble approaches for protein corona study.

中文翻译：

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超分辨率显微镜揭示了纳米粒子蛋白电晕中的动态异质性

血清蛋白的吸附，导致形成生物分子电晕，是体内纳米颗粒生物学特性的关键决定因素。因此，获得有关电晕的形成，组成和时间演变的知识对于开发基于纳米粒子的疗法至关重要。在这里，表明使用超高分辨率光学显微镜可以对具有单一蛋白质敏感性的介孔二氧化硅纳米粒子上的蛋白质电晕成像。逐颗粒定量分析表明在天然条件下蛋白质吸收存在明显的异质性。而且，电晕的多样性随着时间的流逝而发展，这取决于颗粒的表面化学性质和可降解性。本文研究了抗体功能化的纳米颗粒对特定细胞靶向蛋白吸附的后果，从而提供了对电晕活性关系的详细了解。该方法可广泛应用于各种纳米结构，并补充了用于蛋白质电晕研究的现有整体方法。