Native Desorption Electrospray Ionization Liberates Soluble and Membrane Protein Complexes from Surfaces,Angewandte Chemie International Edition

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Native Desorption Electrospray Ionization Liberates Soluble and Membrane Protein Complexes from Surfaces
Angewandte Chemie International Edition ( IF 16.1 ) Pub Date : 2017-09-18 11:33:42 , DOI: 10.1002/anie.201704849
Stephen Ambrose ₁ , Nicholas G. Housden ₂ , Kallol Gupta ₁ , Jieyuan Fan ₁ , Paul White ₂ , Hsin-Yung Yen ₁ , Julien Marcoux _{1,

3} , Colin Kleanthous ₂ , Jonathan T. S. Hopper ₁ , Carol V. Robinson ₁

Affiliation

Folded soluble and membrane proteins can be released directly from surfaces using a desorption electrospray ionization source coupled to a high-resolution mass spectrometer. Desorbed protein complexes maintain non-covalent interactions enabling rapid measurement of subunit stoichiometries and capturing specific ligand binding to a GPCR.

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