A new water-soluble polysaccharide (ESPS-1), with the average molecular weight of 52.1 kDa and a specific optical rotation of +169.7° (c 1.0, H₂O), was successfully isolated by warm-water extraction and then purified using DEAE-Cellulose and Sephadex G-100 columns from the roots of Eremurus stenophyllus. The results from chemical and instrumental tests including FT-IR, methylation and GC–MS analysis, periodate oxidation and smith degradation, partial acid hydrolysis, and NMR spectroscopy (¹H, ¹³C, ¹H-¹H COSY, HMQC and HMBC) demonstrated that ESPS-1 was a 2-O-acetylgalactan, composed of galactose, arabinose and mannose in a molar ratio of about 10:3:1, respectively. It has a backbone of (1 → 6)-α-D-galactopyranosyl residues, with branches at O-3 consisting of α-D-Manp-(1 → 3)-α-L-Araf-(1 → 3)-α-L-Araf-(1 → 3)-α-L-Araf-(1→. The acetyl groups are substituted at O-2 of (1 → 6)-linked Galp and the degree of acetylation was about 14%.

中文翻译：

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面包草根的新水溶性多糖的分离，纯化和结构表征

通过温水萃取成功分离出平均分子量为52.1 kDa，比旋光度为+ 169.7°（c 1.0，H ₂ O）的新型水溶性多糖（ESPS-1），然后使用细叶大戟根的DEAE-纤维素和Sephadex G-100色谱柱。化学和仪器测试的结果，包括FT-IR，甲基化和GC-MS分析，高碘酸盐氧化和史密斯降解，部分酸水解和NMR光谱（¹ H，¹³ C，¹ H- ¹ H COSY，HMQC和HMBC）证明ESPS-1是2- O-乙酰半乳聚糖，分别由半乳糖，阿拉伯糖和甘露糖组成，摩尔比约为10：3：1。它具有（1→6）-α-d吡喃半乳糖基残基，一个主链分支在ö -3选自α-d-曼的p - （1→3）-α-L-阿糖胞苷˚F - （1→3 ）-α-L-阿糖胞苷˚F - （1→3）-α-L-阿糖胞苷˚F - （1→的乙酰基团在取代的Ô -2的（1→6） -连接的半乳糖p和乙酰化度大约是14％。