Poly(pyrrole)-nanowires (PPy-NWs) were efficiently printed by using nano printing on flexible thermoplastics, and then used to device an impedimetric immunosensor. The present pioneering technology allow to create high sensitive and disponsable immunosensor devised using a low cost and simple fabrication. The innovative nanocontact printing uses a PDMS stamp replicated from a CD mold. The PPyNW printing uses controlled chemical polymerization to print PPy-NWs on poly (ethylene terephthalate) and polyether ether ketone surfaces. Atomic force microscopy analysis of PPyNW revealed a width, height and a separation length of 125 ± 8 nm, 377 ± 5 nm and 172 ± 4 nm, respectively. The PPy-NWs were characterized by scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS) and electrochemical impedance spectroscopy (EIS). The XPS results evidenced the covalent bonding between the thermoplastic surface and the PPyNWs. As a final point, the immunosensor was tested for the quantification of Interleukin 6 recombinant human (IL6, Ag) using EIS. The PPy-NWs were functionalized via diazonium coupling reaction and carbodiimide crosslinker chemistry for the immobilization of Anti-human IL-6 monoclonal antibodies (IL6 mAb’s). The developed immunosensor exhibited a sensitivity of 0.013 (pg/mL)⁻¹ (linear fitting at R² = 0.99) and limit of detection (LOD) of 0.36 pg/mL in a linear range of 1–50 pg/mL for Ag IL-6, with a relative standard deviation percentage (RSD%) at 7.6%.

通过在柔性热塑性塑料上进行纳米印刷，可以有效地印刷聚吡咯-纳米线（PPy-NWs），然后将其用于阻抗测定式免疫传感器。当前的先驱技术允许创建使用低成本和简单制造设计的高灵敏度和负责任的免疫传感器。创新的纳米接触印刷使用从CD模具复制的PDMS印章。PPyNW印刷使用受控化学聚合在聚对苯二甲酸乙二酯和聚醚醚酮表面上印刷PPy-NW。PPyNW的原子力显微镜分析显示宽度，高度和分离长度分别为125±8 nm，377±5 nm和172±4 nm。通过扫描电子显微镜（SEM），X射线光电子能谱（XPS）和电化学阻抗能谱（EIS）对PPy-NW进行了表征。XPS结果证明了热塑性表面和PPyNW之间的共价键合。最后，使用EIS测试了免疫传感器对白介素6重组人（IL6，Ag）的定量。通过重氮偶合反应和碳二亚胺交联剂化学作用将PPy-NWs功能化，以固定抗人IL-6单克隆抗体（IL6 mAb）。研发的免疫传感器显示出0.013（pg / mL）的灵敏度^-1（R ²处的线性拟合 = 0.99），对于Ag IL-6，在1–50 pg / mL的线性范围内的检出限（LOD）为0.36 pg / mL，相对标准偏差百分比（RSD％）占7.6％。