One-dimensional bimetallic [email protected] core-shell nanorods (NRs) with uniform Au shell were synthesized and applied to construct a highly sensitive acetylcholinesterase (AChE) biosensor for the detection of organophosphate pesticides (OPs). The [email protected] core-shell nanorods with excellent conductivity, pure contact surface and good biocompatibility can provide an extremely suitable area for the adhesion of AChE. Due to these characteristics, the constructed sensor exhibits a superior performance. The biosensor was prepared via a simple procedure and its optimization studies were discussed. Under optimal conditions, the inhibition of paraoxon (as a model compound for OPs) in linearly proportional to its concentration in the range of 3.6 pM–100 nM, with the detection limit of 3.6 pM (calculated for 10% inhibition). The KMapp value of the fabricated enzyme electrode was calculated to be 0.207 mM. The application of this biosensor in the analysis of real samples was also evaluated. Moreover, the prepared biosensor possessed higher sensitivity and acceptable reproducibility and stability, showing great potential in the detection of OPs.

合成具有均匀金壳的一维双金属[受电子邮件保护的]核壳纳米棒（NRs），并将其用于构建用于检测有机磷酸酯农药（OPs）的高灵敏度乙酰胆碱酯酶（AChE）生物传感器。受[电子邮件保护]的核壳纳米棒具有出色的导电性，纯净的接触表面和良好的生物相容性，可为AChE的粘附提供极为合适的区域。由于这些特性，所构造的传感器表现出优异的性能。通过简单的程序制备了生物传感器，并讨论了其优化研究。在最佳条件下，对氧磷（作为OPs的模型化合物）的抑制作用与其浓度在3.6 pM–100 nM范围内成线性比例，检出限为3.6 pM（针对10％抑制作用计算）。所制造的酶电极的KMapp值经计算为0.207mM。还评估了该生物传感器在实际样品分析中的应用。此外，所制备的生物传感器具有较高的灵敏度，可接受的重现性和稳定性，在检测OP方面显示出巨大的潜力。