A novel biosensor was developed by conjugating Salmonella typhimurium aptamer with the “rotator” ε-subunit of F₀F₁–ATPase within chromatophores through an anti-ε-subunit antibody–biotin–avidin–biotin–aptamer linker to capture bacteria. The bacterial load decreased the rotation rate of F₀F₁-ATPase, which led to the decrease in ATP synthesis. The detection of S. typhimurium was based on proton flux change driven by the F₀F₁-ATPase-mediated ATP-synthesis, which was indicated by fluorescence probe, monitored by a fluorescence spectrometer. Under optimal conditions, the correlation between the relative fluorescence signal intensity and the concentration of S. typhimurium was observed to be linear within the range of 10¹ to 10⁴ cfu·mL⁻¹ (R² = 0.9944). The limit of detection for S. typhimurium was as low as 10 cfu·mL⁻¹. The results demonstrate that the F₀F₁-ATPase molecular motor biosensor can specifically detect S. typhimurium at low concentration, and will be a convenient, quick, and promising tool for detecting pathogens.

通过将鼠伤寒沙门氏菌适体与染色体内F ₀ F ₁ -ATPase的“旋转子”ε亚基通过抗ε亚基抗体-生物素-亲和素-生物素-适体接头结合，开发了一种新型生物传感器。细菌负荷降低了F ₀ F ₁ -ATPase的旋转速率，从而导致了ATP合成的减少。鼠伤寒沙门氏菌的检测基于F ₀ F ₁驱动的质子通量变化-ATP酶介导的ATP合成，由荧光探针指示，由荧光光谱仪监测。在最佳条件下，相对荧光信号强度与鼠伤寒沙门氏菌浓度之间的相关性在10 ¹至10 ⁴ cfu·mL ^-1（R ²  = 0.9944）的范围内呈线性关系。鼠伤寒沙门氏菌的检出限低至10 cfu·mL ^-1。结果表明，F ₀ F ₁ -ATPase分子运动生物传感器可以特异性检测鼠伤寒沙门氏菌。 浓度低，将是检测病原体的方便，快捷和有前途的工具。