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Simultaneous determination of ginsenosides Rg1, Re, and Rb1 and notoginsenoside R1 by solid phase extraction followed by UHPLC-MS/MS and investigation of their concentrations in various kinds of cosmetics
Analytical Methods ( IF 2.7 ) Pub Date : 2017-08-30 00:00:00 , DOI: 10.1039/c7ay01651d Ying Peng 1, 2, 3, 4 , Zhijiang Wu 1, 2, 3, 4 , Yingpeng Huo 1, 2, 3, 4 , Yanwu Chen 1, 2, 3, 4 , Fenghui Lu 1, 2, 3, 4 , Qi Peng 1, 2, 3, 4 , Yingying Liang 1, 2, 3, 4
Analytical Methods ( IF 2.7 ) Pub Date : 2017-08-30 00:00:00 , DOI: 10.1039/c7ay01651d Ying Peng 1, 2, 3, 4 , Zhijiang Wu 1, 2, 3, 4 , Yingpeng Huo 1, 2, 3, 4 , Yanwu Chen 1, 2, 3, 4 , Fenghui Lu 1, 2, 3, 4 , Qi Peng 1, 2, 3, 4 , Yingying Liang 1, 2, 3, 4
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Panax notoginseng saponins, mainly including ginsenosides Rg1 (GRg1), Re (GRe), and Rb1 (GRb1) and notoginsenoside R1 (NR1), have been widely used in cosmetic products due to their various properties such as anti-aging, anti-oxidation and accelerating skin metabolism. In this study, a solid phase extraction combined with an ultrahigh performance liquid chromatography tandem mass spectrometry method has been developed and validated for monitoring the concentrations of GRg1, GRe, GRb1 and NR1 in different kinds of cosmetic products. Pretreatment conditions of samples including the purification column, sample concentration, and washing and elution conditions of a selected column were optimized. Four target analytes were separated on a T3 column by using an optimized gradient elution program, with acetonitrile–water as the mobile phase. The calibration curves were linear in the ranges of 0.05–5 mg L−1 for NR1 and 0.1–10 mg L−1 for GRg1, GRe and GRb1, with correlation coefficients all greater than 0.996. Moreover, the corresponding method limit of detection (MLOD) and the method limit of quantitation (MLOQ) were in the ranges of 0.3–1.0 mg kg−1 and 1.0–3.5 mg kg−1, respectively. Recoveries of four target analytes were in the range of 80.9–93.0% with relative standard deviations (RSDs, n = 6) of 1.9–8.2%. This developed method with excellent linearity and high sensitivity was simple and rapid, and was successfully applied to determine the concentrations of GRg1, GRe, GRb1 and NR1 in various kinds of cosmetic products.
中文翻译：
固相萃取-UHPLC-MS / MS同时测定人参皂苷Rg1，Re和Rb1和三七皂苷R1并同时测定其在各种化妆品中的浓度
三七皂苷主要包括人参皂苷Rg1（GRg1），Re（GRe）和Rb1（GRb1）和三七皂苷R1（NR1），由于它们的多种特性，如抗衰老，抗氧化和加速作用，已广泛用于化妆品中皮肤新陈代谢。在这项研究中，开发了一种固相萃取结合超高效液相色谱串联质谱法的方法，该方法已得到验证，可用于监测不同种类化妆品中GRg1，GRe，GRb1和NR1的浓度。优化了样品的预处理条件，包括纯化柱，样品浓度以及所选柱的洗涤和洗脱条件。使用优化的梯度洗脱程序，以乙腈-水为流动相，在T3色谱柱上分离出四种目标分析物。-1用于NR1和0.1-10毫克的L -1为GRg1，GRE和GRb1，相关系数都大于0.996。此外，相应的检测方法极限（MLOD）和定量方法极限（MLOQ）分别在0.3–1.0 mg kg -1和1.0–3.5 mg kg -1的范围内。四种目标分析物的回收率在80.9–93.0％的范围内，相对标准偏差（RSDs， n = 6）为1.9–8.2％。这种线性度高，灵敏度高的发达方法简便，快速，已成功用于测定各种化妆品中GRg1，GRe，GRb1和NR1的浓度。
更新日期：2017-09-18
中文翻译：
固相萃取-UHPLC-MS / MS同时测定人参皂苷Rg1，Re和Rb1和三七皂苷R1并同时测定其在各种化妆品中的浓度
三七皂苷主要包括人参皂苷Rg1（GRg1），Re（GRe）和Rb1（GRb1）和三七皂苷R1（NR1），由于它们的多种特性，如抗衰老，抗氧化和加速作用，已广泛用于化妆品中皮肤新陈代谢。在这项研究中，开发了一种固相萃取结合超高效液相色谱串联质谱法的方法，该方法已得到验证，可用于监测不同种类化妆品中GRg1，GRe，GRb1和NR1的浓度。优化了样品的预处理条件，包括纯化柱，样品浓度以及所选柱的洗涤和洗脱条件。使用优化的梯度洗脱程序，以乙腈-水为流动相，在T3色谱柱上分离出四种目标分析物。-1用于NR1和0.1-10毫克的L -1为GRg1，GRE和GRb1，相关系数都大于0.996。此外，相应的检测方法极限（MLOD）和定量方法极限（MLOQ）分别在0.3–1.0 mg kg -1和1.0–3.5 mg kg -1的范围内。四种目标分析物的回收率在80.9–93.0％的范围内，相对标准偏差（RSDs， n = 6）为1.9–8.2％。这种线性度高，灵敏度高的发达方法简便，快速，已成功用于测定各种化妆品中GRg1，GRe，GRb1和NR1的浓度。
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