Development of a smart activity-based probe to detect subcellular activity of asparaginyl endopeptidase in living cells,Organic & Biomolecular Chemistry

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Development of a smart activity-based probe to detect subcellular activity of asparaginyl endopeptidase in living cells
Organic & Biomolecular Chemistry ( IF 2.9 ) Pub Date : 2017-09-12 00:00:00 , DOI: 10.1039/c7ob01467h
Jong-Ah Hong _{1,

2,

3,

4} , Na-Eun Choi _{1,

2,

3,

4} , Yeo-Kyoung La _{1,

2,

3,

4} , Ho Yeon Nam _{4,

5,

6,

7} , Jiwon Seo _{4,

5,

6,

7} , Jiyoun Lee _{1,

2,

3,

4}

Affiliation

We developed a smart activity-based probe that detects the activity of asparaginyl endopeptidase (AEP) in live cells to monitor the dynamics of enzyme regulation. The newly designed probe generated a turn-on fluorescence signal in response to the activity of AEP in living cells without compromising the labelling efficiency or selectivity. Our probe closely reflected the enzyme activity in its native state, detecting subcellular AEP activity in colon cancer cells and neuronal cells.

中文翻译：

基于活动的智能探针的开发，可检测活细胞中天冬酰胺基内肽酶的亚细胞活性

我们开发了一种基于活动的智能探针，该探针可检测活细胞中天冬酰胺基内肽酶（AEP）的活性，以监测酶调节的动态。新设计的探针响应活细胞中AEP的活性而产生开启荧光信号，而不会影响标记效率或选择性。我们的探针紧密反映了天然状态下的酶活性，可检测结肠癌细胞和神经元细胞中的亚细胞AEP活性。

更新日期：2017-09-18

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