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Rapid and highly sensitive detection of extracellular and intracellular H2S by an azide-functionalized Al(III)-based metal–organic framework
Dalton Transactions ( IF 3.5 ) Pub Date : 2017-09-01 00:00:00 , DOI: 10.1039/c7dt02293j Soutick Nandi 1, 2, 3, 4 , Helge Reinsch 5, 6, 7, 8 , Sooram Banesh 2, 4, 9, 10 , Norbert Stock 5, 6, 7, 8 , Vishal Trivedi 2, 4, 9, 10 , Shyam Biswas 1, 2, 3, 4
Dalton Transactions ( IF 3.5 ) Pub Date : 2017-09-01 00:00:00 , DOI: 10.1039/c7dt02293j Soutick Nandi 1, 2, 3, 4 , Helge Reinsch 5, 6, 7, 8 , Sooram Banesh 2, 4, 9, 10 , Norbert Stock 5, 6, 7, 8 , Vishal Trivedi 2, 4, 9, 10 , Shyam Biswas 1, 2, 3, 4
Affiliation
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A new, azide-functionalized Al(III)-based metal–organic framework (MOF) denoted as CAU-10-N3 (1, CAU = Christian-Albrechts-University) and consisting of the 5-azido-isophthalic acid (H2IPA-N3) ligand was employed as a reaction-based fluorescent turn-on probe for the detection of H2S. The activated compound (1′) showed fast, selective and highly sensitive sensing properties for extracellular H2S in HEPES buffer (10 mM, pH = 7.4). The material retained its high selectivity even in the presence of possibly competing biological species. The limit of detection of 1′ for H2S is 2.65 μM, which is lower than the earlier reports on MOFs for H2S sensing. The material displayed a short response time (420 s) and a significant increase (20-fold and 26-fold after 1 and 7 min of addition of Na2S, respectively) in the fluorescence intensity towards H2S. Macrophage cells loaded with probe 1′ exhibited blue fluorescence with a response time of 15 min after Na2S addition, indicating the suitability of the probe for intracellular H2S detection. Moreover, CAU-10-N3 featured excellent detection performance (quick response and 32-fold increment in fluorescence intensity after 7 min of Na2S addition) in water. Hence, it can be utilized to regulate the H2S level in aqueous samples collected from the environment.
中文翻译：
叠氮化物官能化的基于Al（III）的金属-有机框架对细胞外和细胞内H 2 S的快速和高灵敏度检测
一种新的叠氮化物官能化的基于Al（III）的金属-有机骨架（MOF），表示为CAU-10-N 3（1，CAU = Christian-Albrechts-University），由5-叠氮基间苯二甲酸（H 2 IPA-N 3）配位体被用作一个反应为基础的荧光接通探针用于检测H的2 S的活化的化合物（1' ）显示速度快，对胞外ħ选择性和高灵敏度的传感特性2 S IN HEPES缓冲液（10 mM，pH = 7.4）。即使在可能存在竞争生物物种的情况下，该材料仍保持其高选择性。H 2的1'检出限S为2.65μM，低于先前有关H 2 S感应的MOF的报告。该材料对H 2 S的荧光强度显示出较短的响应时间（420 s）和显着增加（分别在添加Na 2 S 1和7分钟后分别增加20倍和26倍）。Na 2 S加入后，探针1'呈现蓝色荧光，响应时间为15分钟，表明该探针适用于细胞内H 2 S检测。此外，CAU-10-N 3具有出色的检测性能（Na 2 7分钟后反应迅速，荧光强度增加了32倍）S另外）在水中。因此，它可用于调节从环境中收集的水性样品中的H 2 S含量。
更新日期：2017-09-18
中文翻译：
叠氮化物官能化的基于Al（III）的金属-有机框架对细胞外和细胞内H 2 S的快速和高灵敏度检测
一种新的叠氮化物官能化的基于Al（III）的金属-有机骨架（MOF），表示为CAU-10-N 3（1，CAU = Christian-Albrechts-University），由5-叠氮基间苯二甲酸（H 2 IPA-N 3）配位体被用作一个反应为基础的荧光接通探针用于检测H的2 S的活化的化合物（1' ）显示速度快，对胞外ħ选择性和高灵敏度的传感特性2 S IN HEPES缓冲液（10 mM，pH = 7.4）。即使在可能存在竞争生物物种的情况下，该材料仍保持其高选择性。H 2的1'检出限S为2.65μM，低于先前有关H 2 S感应的MOF的报告。该材料对H 2 S的荧光强度显示出较短的响应时间（420 s）和显着增加（分别在添加Na 2 S 1和7分钟后分别增加20倍和26倍）。Na 2 S加入后，探针1'呈现蓝色荧光，响应时间为15分钟，表明该探针适用于细胞内H 2 S检测。此外，CAU-10-N 3具有出色的检测性能（Na 2 7分钟后反应迅速，荧光强度增加了32倍）S另外）在水中。因此，它可用于调节从环境中收集的水性样品中的H 2 S含量。
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