Bioactive and antibacterial coatings on stainless steel substrates were developed and characterized in this study. Silver nanocluster–silica composite coatings of 60–150 nm thickness were deposited using radio frequency (RF) co-sputtering on PEEK/bioactive glass (BG) layers (of 80–90 μm thickness) which had been electrophoretically deposited onto stainless steel. Two sputtering conditions were used by varying the deposition time (15 and 40 min); the resulting microstructure, composition, adhesion strength, in vitro bioactivity, and antibacterial activity were investigated. Scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), and energy dispersive spectroscopy (EDX) confirmed the presence of silver nanoclusters, which were homogeneously embedded in the silica matrix. The isoelectric point of the coatings and their charge at physiological pH were determined by zeta potential measurements. The presence of BG particles in the PEEK/BG layer allows the coatings to form apatite-like crystals upon immersion in simulated body fluid (SBF). Moreover, silver nanoclusters embedded in the silica matrix as a top layer provided an antibacterial effect against Escherichia coli and Staphylococcus carnosus.

中文翻译：

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基于射频共溅射在电泳沉积获得的PEEK /生物活性玻璃层上的银纳米团簇-二氧化硅涂层的抗菌和生物活性涂层

本研究开发并表征了不锈钢基材上的生物活性和抗菌涂层。使用射频（RF）共溅射在PEEK /生物活性玻璃（BG）层（厚度为80-90μm）上沉积厚度为60-150 nm的银纳米簇-二氧化硅复合涂层，该层已电泳沉积在不锈钢上。通过改变沉积时间（15和40分钟）使用了两种溅射条件。研究了所得的微观结构，组成，粘附强度，体外生物活性和抗菌活性。扫描电子显微镜（SEM），X射线光电子能谱（XPS）和能量色散能谱（EDX）证实了银纳米团簇的存在，它们均匀地嵌入二氧化硅基质中。通过ζ电势测量来确定涂层的等电点及其在生理pH下的电荷。PEEK / BG层中BG颗粒的存在使涂层浸入模拟体液（SBF）后形成磷灰石样晶体。此外，嵌入在二氧化硅基质中作为顶层的银纳米团簇提供了对以下细菌的抗菌作用大肠杆菌和金黄色葡萄球菌。