Salamanders are unparalleled among tetrapods in their ability to regenerate many structures, including entire limbs, and the study of this ability may provide insights into human regenerative therapies. The complex structure of the limb poses challenges to the investigation of the cellular and molecular basis of its regeneration. Using CRISPR/Cas, we genetically labelled unique cell lineages within the developing axolotl embryo and tracked the frequency of each lineage within amputated and fully regenerated limbs. This allowed us, for the first time, to assess the contributions of multiple low frequency cell lineages to the regenerating limb at once. Our comparisons reveal that regenerated limbs are high fidelity replicas of the originals even after repeated amputations.

中文翻译：

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基因组编辑等位基因的谱系追踪揭示了高保真蝾螈肢体再生

蝾螈的再生能力在四足动物中是无与伦比的，包括整个四肢，对这种能力的研究可能会为人类再生疗法提供见解。肢体的复杂结构对其再生的细胞和分子基础的研究提出了挑战。使用 CRISPR/Cas，我们对发育中的蝾螈胚胎内的独特细胞谱系进行了基因标记，并跟踪了截肢和完全再生肢体中每个谱系的频率。这使我们第一次能够同时评估多个低频细胞谱系对再生肢体的贡献。我们的比较表明，即使经过反复截肢，再生的肢体也是原始肢体的高保真复制品。